Bovine Anaplasma Antibody Rapid Test

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INTENDED USE

The Bovine Anaplasma Antibody Rapid Test is a test cassette to diagnose the presence of Anaplasma spp. antibodies in cattle’s blood specimen.

Assay Time: 5-10 minutes

Specimen: Serum, plasma, whole blood

PRINCIPLE

The Bovine Anaplasma Antibody Rapid Test is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into the sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated Anaplasma recombinant antigens. If there are Anaplasma antibodies in the specimen, a visible T line will appear. The C line should always appear after a sample is applied, which indicates a valid result. By this means, the device can accurately indicate the presence of Anaplasma antibodies in the specimen.

REAGENTS AND MATERIALS

20 Test devices
1 Bottle of Assay buffer
20 Capillary droppers
1 Products Manual

STORAGE AND STABILITY

The kit can be stored at room temperature (4-30°C). The test kit is stable through the expiration date (18 months) marked on the package label. DO NOT FREEZE. Do not store the test kit in direct sunlight.

SPECIMEN PREPARATION AND STORAGE

Specimen should be obtained and treated as below.

Serum or plasma: collect the whole blood for the patient cattle, centrifuge it to get the plasma, or place the whole blood into a tube which contains anticoagulants to get serum.
Whole blood: collect fresh blood for use directly or make anticoagulant blood for storage at 2-8℃.

All specimen should be tested immediately. If not for testing right now, they should be stored at 2-8℃.

TEST PROCEDURE

Allow all materials, including specimen and test device, recover to 15-25℃ before running the assay.

Take out the test device from the foil pouch and place it Using the capillary dropper to place 10μL of the prepared specimen into the sample hole “S” of the test device.Then place 3 drops of the assay buffer into the sample hole “S” immediately.