Bovine Cryptosporidium Antigen Rapid Test

INTENDED USE

Cryptosporidium is a common intestinal parasite that affects not only bovines, cats, dog, but also human beings. It can cause symptoms like diarrhea, vomiting, gas, and abdominal discomfort in animals. The Bovine Cryptosporidium Antigen Rapid Test is a lateral flow immunochromatographic assay for the qualitative detection of Cryptosporidium antigen (Crypto Ag) in cattle’s feces or vomit specimen.

Assay Time:  5-10 minutes

Specimen: feces or vomit

PRINCIPLE

The Bovine Cryptosporidium Antigen Rapid Test is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into the sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated monoclonal antibodies. If there is Cryptosporidium antigen in the specimen, a visible T line will appear. The C line should always appear after a sample is applied, which indicates a valid result. By this means, the device can accurately indicate the presence of Cryptosporidium antigen in the specimen.

REAGENTS AND MATERIALS

• 20 Test devices, with disposable droppers
• 20vials of Assay buffer
• 20 Swabs
• 1 Products Manual

STORAGE AND STABILITY

The kit can be stored at room temperature (4-30°C). The test kit is stable through the expiration date (18 months) marked on the package label. DO NOT FREEZE. Do not store the test kit in direct sunlight.

TEST PROCEDURE

• Collect cattle’s fresh feces or vomit with the swab from bovine’s anus or from the ground.  
• Insert the swab into the provided assay buffer tube. Agitate it to get efficient sample extraction.
• Allow all materials, including specimen and test device, recover to 15-25℃ before running the assay.
• Take out the test device from the foil pouch and place it horizontally.
• • Suck the treated sample extraction from the assay buffer tube and place 4drops into the sample hole “S” of the test device.

  Note: If the liquid will not flow through the surface of the test strip in 30 seconds, please add another drop of the treated sample extraction.

  • Interpret the result in 5-10 minutes. Result after 15minutes is considered as invalid.

  INTERPRETATION OF RESULTS