Bovine Escherichia coli K99 Antigen Rapid Test
INTENDED USE
The Bovine Escherichia coli K99 Antigen Rapid Test is a lateral flow immunochromatographic assay for the qualitative detection of Escherichia coli K99 antigen (E.coli K99 Ag) in cattle’s feces specimen.
Assay Time: 5-10 minutes
Specimen: feces
PRINCIPLE
The Bovine Escherichia coli K99 Antigen Rapid Test is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into the sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated monoclonal antibodies. If there is Escherichia coli K99 antigen in the specimen, a visible T line will appear. The C line should always appear after a sample is applied, which indicates a valid result. By this means, the device can accurately indicate the presence of Escherichia coli K99 antigen in the specimen.
INTERPRETATION OF RESULTS
- Positive (+): The presence of both “C” lineand zone “T” line, no matter T line is clear or vague.
- Negative (-): Only clear C line appears. No T line.
- Invalid:No colored line appears in C zone. No matter if T line appears.
