Bovine Tuberculosis Antibody Rapid Test

Bovine Tuberculosis Antibody Rapid Test

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INTENDED USE

The Bovine Tuberculosis Antibody Rapid Test is a lateral flow immunochromatographic assay for the qualitative detection of Bovine Tuberculosis Antibody (BTB Ab) in cattle’s serum, plasma. MPB70 and MPB83 chimera recombinant was applied in this assay.  

Assay Time:  5-10 minutes

Specimen: serum, plasma

PRINCIPLE

The Bovine Tuberculosis Antibody Rapid Test is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into the sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated Tuberculosis antigens. If there are anti-TB antibodies in the specimen, a visible T line will appear. The C line should always appear after a sample is applied, which indicates a valid result. By this means, the device can accurately indicate the presence of Bovine Tuberculosis antibodies (BTB Ab) in the specimen.

REAGENTS AND MATERIALS

  • 20 Test devices
  • 1 Bottle of Assay Buffer
  • 20 Capillary droppers
  • 1 Products Manual

STORAGE AND STABILITY

The kit can be stored at room temperature (4-30°C). The test kit is stable through the expiration date (18 months) marked on the package label. DO NOT FREEZE. Do not store the test kit in direct sunlight.

TEST PROCEDURE

  • Collect cattle’s fresh whole blood, and get the serum or plasmaspecimen for use. Immediately apply the testing once collected the specimen.
  • Allow all materials, including specimen and test device, recover to 15-25℃before running the assay.
  • Take out the test devicefrom the foil pouch and place it horizontally.
    • Place 1drop (around 10μL) of serum or plasma specimen into the sample hole”S”. Then place 3 drops of assay buffer into the sample hole immediately.
    • Interpret the result in 5-10 Result after 15 minutes is considered as invalid.

    INTERPRETATION OF RESULTS

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