Bovine Viral Diarrhea Virus Antibody Rapid Test
INTENDED USE
The Bovine Viral Diarrhea Virus Antibody Rapid Test (BVDV Ab) is a lateral flow immunochromatographic assay for the qualitative detection of BVDV antibodies in cattle’s serum, plasma. E2 protein recombinant was applied in this assay, for the monitoring of BVDV antibody level and providing an important reference for the vaccination plan.
Assay Time: 5-10 minutes
Specimen: serum, plasma
PRINCIPLE
The Bovine Viral Diarrhea Virus Antibody Rapid Test is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into the sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated BVDV antigens. If there are BVDV antibodies in the specimen, a visible T line will appear. The C line should always appear after a sample is applied, which indicates a valid result.
REAGENTS AND MATERIALS
- 20 Test devices, with disposable droppers
- 1 Bottle of Assay buffer
- 1 Products Manual
STORAGE AND STABILITY
The kit can be stored at room temperature (4-30°C). The test kit is stable through the expiration date (18 months) marked on the package label. DO NOT FREEZE. Do not store the test kit in direct sunlight.
TEST PROCEDURE
- Collect cattle’s fresh whole blood, and get the serum or plasmaspecimen for use. Immediately apply the testing once collected the specimen.
- Allow all materials, including specimen and test device, recover to 15-25℃ before running the assay.
- Take out the test devicefrom the foil pouch and place it horizontally.
- Using the disposabledropper to place 1 drop (approx. 40μL) of the prepared specimen into the sample hole “S” of the test device. Then drop 2 drops (approx. 80μL) of the assay buffer into the sample hole immediat
Interpret the result in 5-10 minutes. Result after 15 minutes is considered as invalid.
INTERPRETATION OF RESULTS
