Canine Distemper Virus Antibody Rapid Test

• INTENDED USE

The Canine Distemper Virus Antibody Rapid Test is a lateral flow immunochromatographic assay for the semi-quantitative detection of Canine Distemper Virus Antibody (CDV Ab) in dog’s serum or plasma.

Assay Time:  10 minutes

Specimen: serum or plasma.

• PRINCIPLE

The Canine Distemper Virus Antibody Rapid Test is based on sandwich lateral flow immunochromatographic assay.

• REAGENTS AND MATERIALS
• Foil pouches (each contains one test cassette,

one disposable dropper and one desiccant)

• Products Manual
• STORAGEAND STABILITY

The kit can be stored at room temperature (4-30°C). The test kit is stable through the expiration date (24 months) marked on the package label. DO NOT FREEZE. Do not store the test kit in direct sunlight.

• SPECIMEN PREPARATION AND STORAGE
• Specimen should be obtained and treated as below.
• Serum or plasma: collect the whole blood for the patient dog, centrifuge it to get the serum, or place the whole blood into a tube which contains anticoagulants to get plasma.
• All specimen should be tested immediately. If not for testing right now, they should be stored at 2-8℃.
• TEST PROCEDURE
• Allow all materials, including specimen and test device, recover to 15-25℃ before running the assay.
• Take out the test device from the foil pouch and place it horizontally.
• Drop 3 drops of the serum or plasma specimen into the sample hole “S” of the test device.
• Interpret the result in 10 minutes. Result after 15 minutes is considered as invalid.
• INTERPRETATION OF RESULTS

The presence of “C” line indicates a valid result. Read the result by comparing the color intensity of the “T” line with the standard color reference scale (from “G1” to “G6”). The V.N.

titers of canine distemper antibodies are corresponding with the color intensity grades.

• Positive (+): The presence of both “C” lineand zone “T” line. T line at G3 is considered as standard positive. T line < G3, is weak positive and indicates low CDV antibody level. T line around G4-G5, is strong positive. T line > G6, is very strong positive, indicates a contact with virus recently or in infection.
• Negative (-): Clear C line appears. No T line, or T line is weaker than G1. It indicates very low CDV titer. Failed immunization may happen.   
• Invalid: No colored line appears in C zone.
• PRECAUTIONS
• All reagents must be at room temperature before running the assay.
• Do not remove test cassette from its pouch until immediately before use.
• Do not use the test beyond its expiration date.
• The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers.
• All specimens are of potential infection. It must be strictly treated according to the rules and regulations by local states.
• LIMITATION

Canine Distemper Virus Antibody Rapid Test is for in vitro veterinary diagnosis use only. All result should be considered with other clinical information available with veterinarian.