FELINE FPV-FHV-FCV Ab COMBO RAPID TEST

INTENDED USE

The Feline FPV-FHV-FCV Ab Combo Rapid Test

 is a lateral flow immunochromatographic assay for the semi-quantitative detection of FPV/FHV/FCV Ab in cat’s serum or plasma.

Assay Time:  10 minutes

Specimen: serum or plasma.

• PRINCIPLE

The FPV-FHV-FCV Ab Combo Rapid Test is based on sandwich lateral flow immunochromatographic assay. The test card has two testing windows for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the sample was applied into the sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated recombinant antigens. If there is FCV, FHV or FPV antibody in the specimen, a visible T line will appears. The C line should always appear after a sample is applied, which indicates a valid result. In this way, the device can accurately indicate the presence of Calicivirus antibody, Herpesvirus Type-1 antibody, Panleukopenia virus antibody in the specimen.

• REAGENTS AND MATERIALS
• Foil pouches (each contains one test cassette,

one disposable dropper and one desiccant)

• Products Manual
• 
• STORAGEAND STABILITY

The kit can be stored at room temperature (4-30°C). The test kit is stable through the expiration date (24 months) marked on the package label. DO NOT FREEZE. Do not store the test kit in direct sunlight.

• TEST PROCEDURE
• Allow all materials, including specimen and test device, recover to 15-25℃ before running the assay.
• Take out the test devicefrom the foil pouch and place it horizontally.
• Drop 3 drops of the serum or plasma specimen into the sample hole “S” of the test device.
• Interpret the result in 10 minutes. Result after 15 minutes is considered as invalid.
• 
  • INTERPRETATION OF RESULTS

• The presence of “C” line indicates a valid result. Read the result by comparing the color intensity of the “T” line with the standard color reference scale (from “G1” to “G6”).
  

  The titers of FPV Ab are corresponding with the color intensity grades.

  G1 < 1:40	G2 = 1:40	G3 = 1:80
  G4 = 1:160	G5 = 1:320	G6 > 1:640

  The titers of FHV Ab are corresponding with the color intensity grades.

  G1 < 1:4	G2 = 1:8	G3 = 1:16
  G4 = 1:32	G5 = 1:64	G6 > 1:128

  The titers of FCV Ab are corresponding with the color intensity grades.

  G1 < 1:8	G2 = 1:16	G3 = 1:32
  G4 = 1:64	G5 = 1:128	G6 > 1:256

  • Positive (+): The presence of both “C” line and zone “T” T line at G3 is considered as standard positive. T line < G3, is weak positive and indicates low FCV, FHVor FPV antibody level. T line around G4-G5, is strong positive. T line > G6, is very strong positive, indicates a contact with virus recently or in infection.
  • Negative (-): Clear C line appears. No T line, or T line is weaker than G1. It indicates very low FCV, FHVor FPV antibody level. Failed immunization may happen.   
  • Invalid: No colored line appears in C zone.
  • PRECAUTIONS
  • All reagents must be at room temperature before running the assay.
  • Do not remove test cassette from its pouch until immediately before use.
  • Do not use the test beyond its expiration date.
  • The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers.
  • All specimens are of potential infection. It must be strictly treated according to the rules and regulations by local states.
  • LIMITATION

• FPV-FHV-FCV Ab Combo Rapid Test is for in vitro veterinary diagnosis use only. All result should be considered with other clinical information available with veterinarian.