SARS - COV - 2 Anti - RBD ya yi karo da antiting

Amfani da aka yi niyya

SARS - COV - 2 anti - RBD da ke ba da gwajin rigakafin ƙwaƙwalwa mai saurin rigakafi ga SARS - COV - 2 A cikin jini, magani, ko plasma.

Abubuwan haɗin

Kayan da aka bayar

• Tsare poiil, tare da kaset na gwaji
• Katin Calibratrat (don ƙidaya)
• Assay buffer
• Jini lance don amfani guda
• Capillary fari
• Pays barasa
• Koyarwar don amfani

Abubuwan da ake buƙata amma ba a bayar ba

• Mocropopette da tukwici (don ƙidaya)
• Karancin gwajin mai sauri (don ƙarin bayani)
• Mai ƙidali

Hanya gwaji

Bada izinin na'urar gwajin, samfur, mai ɗorewa, da / ko sarrafawa don daidaita yanayin zafin jiki (15 - 30 ° C) kafin gwaji.

1. Ku zo da jakar don zazzabi a kafin buɗe. Cire na'urar gwajin daga aljihun da aka rufe kuma amfani da shi da wuri-wuri.
2. Sanya na'urar gwajin a kan tsaftace da kwance.
3. Don Serum ko samfuran Plasma (samfurori):

Yi amfani da pipette don tattara magani ko plasma. Yi amfani da bututun don canja wurin 10ml na samfuran gwajin cikin samfuran gwajin, sai a ƙara daidai 90ml na mai ɗaukar hoto sosai kuma fara lokacin.

1. Don yatsun yatsa gaba ɗaya samfuran jinin jini (ƙididdigar):

Don amfani da micropipette: Riƙe buttette a tsaye a shafin hasashe, tsotse jiki kai tsaye da kuma sanya daidai 90 μL na mai ɗorawa cikin samfuran da kyau kuma fara lokacin .

1. Don Serum ko samfuran Plasma (mai cancanta):

Yi amfani da tsawa don tattara maganin ko plasma. Yi amfani da digo don sanya 1 digo (kimanin.10ml) na samfuran gwajin, sannan ƙara 3 saukad da shi (kimanin. 90mL) na mai ɗaukar hoto sosai kuma fara lokacin.

1. Don yatsun yatsa gaba ɗaya samfuran ji

Don amfani da tsawa mai laushi: riƙe droper a tsaye a kan shafin hasashe, da kuma canja wurin na'urar duka (kimanin 20 μL) cikin samfuran gwaji (kimanin 20 μL) cikin samfuran (kimanin 90 90 μL ) Kuma fara lokacin.

3. Jira layin launi (s) ya bayyana. Karanta sakamako a minti 10. Kada ku fassara sakamakon bayan mintina 15.

Fassarar sakamako

Tabbatacce: Biyu masu launin launuka biyu suna bayyana akan membrane. Bandungiya ɗaya ta bayyana a yankin sarrafawa (c) da wani ƙungiyar ya bayyana a cikin yankin gwajin (t).

Norantarwa: Bunksaye ɗaya ne kawai ya bayyana a yankin sarrafawa (c).Babu wani Band Band Band ya bayyana a cikin yankin gwajin (t).

Ba daidai ba: Bandungiyar sarrafawa ta gaza bayyana.Sakamako daga kowane gwaji wanda bai samar da ƙungiyar sarrafawa ba a lokacin karantawa dole ne a jefar da shi. Da fatan za a duba hanya kuma maimaita tare da sabon gwaji. Idan matsalar ta ci gaba, dakatar da yin amfani da kit ɗin nan da nan kuma tuntuɓi mai raba gida.

1. Dangi mai hankali, takamaiman da daidaito

SARS - COV - 2 anti - RBD ta yanke shawarar gwajin antitody mai saurin gwaji tare da samfurori da aka samu daga yawan samfuran kirki da rashin inganci. Sakamakon ya tabbatar da sakamakon da Sarrs na kasuwanci - Cov - 2 tsinkaye na Kit ɗin Anibody (Elisa Kit, Castodf 30% incibanion siginar).

Dangi na dangi: 95.04% (95% ci: 89.38% ~ 97.93%)

Dangi da dama: 98.84% (95% ci: 96.49% ~ 99.77%)

Tabbatarwa: 97.63% (95% ci: 95.49% ~ 98.82%)