Methamphetamine (MET) Rapid Test (Urine)

Methamphetamine is an addictive stimulant drug that strongly activates certain systems in the brain. Methamphetamine is closely related chemically to Amphetamine, but the central nervous system effects of Methamphetamine are greater. Methamphetamine is made in illegal laboratories and has a high potential for abuse and dependence. The drug can be taken orally, injected, or inhaled. Acute higher doses lead to enhanced stimulation of the central nervous system and induce euphoria, alertness, reduced appetite, and a sense of increased energy and power. Cardiovascular responses to Methamphetamine include increased blood pressure and cardiac arrhythmias. More acute responses produce anxiety, paranoia, hallucinations, psychotic behavior, and eventually, depression and exhaustion.

The effects of Methamphetamine generally last 2-4 hour, and the drug has a half-life of 9-24 hours in the body. In urines of normal pH approximately 43% of a dose is eliminated as unchanged methamphetamine in a 24hour period, with about 4-7% eliminated as amphetamine. Thus, the presence of the parent compound in the urine indicates Methamphetamine use. Methamphetamine is generally detectable in the urine for 3-5 days, depending on urine pH level.

TEST PROCEDURE

Bring tests, specimens, buffer and/or controls to room temperature (1530°C) before use.

1. 1. Remove the test from its sealed pouch, or remove one strip from the canister, and use it as soon as possible. For best results, the assay should be performed within one hour.  Canisters should be closed tightly after removing strips.
2. 2. Hold the strip by the end, where the product name is printed. To avoid contamination, do not touch the strip membrane.
3. 3. Holding the strip vertically, dip the test strip in the urine specimen for at least 10-15 seconds. Do not immerse past the maximum line (MAX) on the test strip.
4. 4. After the test has finished running, remove the strip from the specimen and place it on a non-absorbent flat surface. Start the timer and wait for the colored band(s) to appear. The result should be read at 5 minutes. 5. Do not interpret the result after 8 minutes.

5. INTERPRETATION OF RESULTS
   

   POSITIVE: Only one colored band appears, in the control region (C). No apparent colored band appears in the test region (T).

    

   NEGATIVE: Two colored bands appear on the membrane. One band appears in the control region (C) and another band appears in the test region (T).

    

   INVALID: Control band fails to appear. Results from any test which has not produced a control band at the specified read time must be discarded. Please review the procedure and repeat with a new test. If the problem persists, discontinue using the kit immediately and contact your local distributor.

   NOTE:

   1. The intensity of color in the test region (T) may vary depending on the concentration of analytes present in the specimen. Therefore, any shade of color in the test region should be considered positive. Note that this is a qualitative test only, and cannot determine the concentration of analytes in the specimen.
   2. Insufficient specimen volume, incorrect operating procedure or expired tests are the most likely reasons for control band failure.

   3. MATERIALS

      Materials Provided

      · Test strips (individually pouched or in canisters

      ·  Package insert

      Materials Required but Not provided

      · Positive and negative controls	· Timer
      · Centrifuge

   4. LIMITATIONS OF THE TEST
      

      1. 1. The Methamphetamine (MET) Rapid Test(Urine) is for professional in vitro diagnostic use, and should be only used for the qualitative detection of Methamphetamine.
      2. 2. This assay provides a preliminary analytical test result only. A more specific alternative chemical method must be used in order to obtain a confirmed analytical result. Gas chromatography/mass spectrometry (GC/MS) has been established as the preferred confirmatory method by the National Institute on Drug Abuse (NIDA). Clinical consideration and professional judgment should be applied to any test result, particularly when preliminary positive results are indicated.
      3. 3. There is a possibility that technical or procedural errors as well as other substances and factors may interfere with the test and cause false results.
      4. 4. Adulterants, such as bleach and/or alum, in urine specimens may produce erroneous results regardless of the analytical method used. Therefore, please preclude the possibility of urine adulteration prior to testing.
      5. 5. A positive result indicates the presence of a Methamphetamine only, and does not indicate or measure intoxication.
      6. 6. A negative result does not at any time rule out the presence of Methamphetamine in urine, as they may be present below the minimum detection level of the test.
      7. 7. This test does not distinguish between Methamphetamine and certain medications.
      8. 8. The test results can be used to provide evidence and basis for therapy and treatment plans of drug dependence and toxic psychosis. For laboratory professional in vitro diagnostic use only.
      9. 9. The test should only be performed by health professionals in a clinical/hospital setting to aid in screening of drug of abuse to determine the follow-up treatment measures in combination of clinical symptoms.
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