Bovine Leishmania antibody kuyesa
Kugwiritsa Ntchito
Mayeso a a Bovine Lehishmania antibody amayesa masewera olimbitsa thupi kuti adziwe kupezeka kwa leishmania antibody (LHSH ACTO) m'magazi a ng'ombe.
Nthawi Yachikulu: 5 - Mphindi 10
Fanizo: seramu, plasma, magazi athunthu
Maganizo
Mayeso a a Bovine Lehishmania antibody amakhazikitsidwa pa sangweji yotsatira yotsatira Imnochrogictict.
Ma reagents ndi zida
- Zida 20 zoyeserera
- 1 botolo la stay buffer
- 20 capillary madontho
- 1 Zolemba
Kusunga Ndi kukhazikika
Kit ikhoza kusungidwa mu kutentha kwa firiji (4 - 30 ° C). Makina oyesererawo ndi okhazikika kudzera tsiku lotha ntchito (miyezi 18) chizindikiro pa phukusi la zilembo.Osamazizira. Osasunga zoyeserera zoyeserera.
Kukonzekera kwa Cerekena ndi Kusunga
- Fanizo liyenera kupezeka ndi kuthandizidwa monga pansipa.
- Seramu kapena plasma: Sonkhanitsani magazi athunthu kwa ng'ombe zodwala, centrifuge kuti mutenge seramu, kapena kuyika magazi athunthu mu chubu chomwe chili ndi anticoagulants kuti atenge mankhwala.
- Magazi athunthu: Sungani magazi atsopano kuti mugwiritse ntchito mwachindunji kapena kupanga magazi a anticoagulant kuti musungidwe 2 - 8 ℃.
- Zolinga zonse ziyenera kuyesedwa nthawi yomweyo. Ngati sichoncho poyesa pakali pano, ayenera kusungidwa pa 2 - 8 ℃.
Njira Yoyeserera
- Lolani zida zonse, kuphatikizapo chida choyeserera ndi mayeso, kuchira kwa zaka 15 - lisanayambe kuyika.
- Tengani chida choyesera kuchokera ku thumba la zondimba ndikuziyika molunjika.
- Pogwiritsa ntchito kapilala komwe kumayambira 10μfu yokonzekereratu kulowa m'mphepete mwa chipinda cha mayeso .Ndin Drop 3 ya chipangizocho.
- Tanthauzirani zotsatira za 5 - zotsatira 10 pambuyo pa ma 15minate zimatengedwa ngati zosavomerezeka.
-
Kutanthauzira kwa Zotsatira
