Clostridium Hardile Antigen mwachangu mayeso

Kugwiritsa Ntchito

Clostridium Hardile. Zotsatira zoyesedwa zakonzedwa kuti zithandizire kupeza maantikusioti - omwe amaphatikizidwa ndi matenda a m'mimba ndi pseudomembranous colitis yoyambitsidwa ndi matendaClostridium Hardile.

Maganizo

Pogwiritsa ntchito mfundo ya Colloidal Golide Imnochromatography, got anti - mbewa ya polyclonal antibody (mndandanda c) ndi mbewa anti -Clostridium HardileOgwiritsa ntchito monolol anali kuthiridwa mu fitture cellulose. Mbewa monoclodies kuC. Hardadakhazikika pa mbale zagolide ndi zilembo zagolide za Colloidal. Pamene zitsanzo zabwino zimayesedwa,Clostridium HardileAntigen mu chitsanzo chimayamba ku mbewaClostridium HardileAntibodnt antibody pa pad wagolide, ndikupanga zovuta zomwe zimayenda mu membrane kudzera mu chromatography. Pambuyo pa mzere wowoneka bwino, adapanga Sangweyi

Njira Yoyeserera

Bweretsani Ziyeso, Zoyesa, ndi / kapena Kuwongolera Kutentha Kwachipinda (15 - 30 ° C)musanagwiritse ntchito.

1. Chotsani mayesowo m'thumba lake losindikizidwa, ndikuyika pa oyera pamtunda. Lembani chipangizocho ndi chizindikiritso kapena chowongolera. Zotsatira zabwino kwambiri zomwe zikuyenera kuchitika mkati mwa ola limodzi.

2. Kukonzekera kwa Dengo:

Omwe atulutsire kapu ya chubu cholumikizira, ndiye kuti mwachisawawa ndodo yopachika mu stool stool pamasamba atatu osiyanasiyana kuti atole ma stool (ofanana ndi 1/4 ya mtola). Gwirani machubu operekera machubu ophatikizira ndi cholembera chopindika, ikani ndodo ya zitsanzo, Finyani pansi pa chubu. Sakanizani stool colol ndi buffer bwino bwino pogwedeza botolo kwa masekondi angapo.

3. Njira Yabwino:

- Kupirira kapu pa chubu cholumikizira, kenako gwedezani chubu cholumikizira moyenera kusakaniza fanizo ndi thunthu la m'zigawo. Siyani chubu chokha kwa mphindi ziwiri.

Kuchotsa chivundikiro chaching'ono pamwamba.

- Gwirani botolo limodzi lokhazikika pa chiwonetsero cha chipangizochi, ikani madontho atatu (pafupifupi 90μl) ya Stool Stool to the stool ndikuyambitsa nthawi.

Zindikirani:Pewani kutchera ma thovu a mpweya mu chinsalu (s), ndipo osawonjezera njira iliyonse yothandizira.

Pamene mayeso amayamba kugwira ntchito, utoto umasamukira kudera lomwe lili pakatikati pa chipangizocho.

Kudikirani gulu lauda kuti liziwoneka. Werengani zotsatira za 5 - Mphindi 10. Chitsanzo chabwino champhamvu chitha kuwonetsa zotsatira zoyambirira. Osatanthauzira zotsatira pambuyo 15 mphindi. Pomwe mayeso amayamba kugwira ntchito, utoto

Kusamukira kudera lomwe lili pakatikati pa chipangizocho.

Kutanthauzira kwa Zotsatira

Zabwino (+):Magulu awiri ofiira amawoneka. Imodzi ili m'dera lofufuzira (T), linalo limapezeka m'chigawo chowongolera (c).

Zindikirani:Gulu lofiirira lofiirira m'dera lofufuzira (T) limatha kuwonetsa chodabwitsa cha mtundu wamdima ndi wopepuka. Komabe, panthawi yomwe mwapezedwa, mosasamala mtundu wa gulu, ngakhale gulu lofooka kwambiri

ziyenera kutanthauziridwa kuti ndi zotsatira zabwino.

Zoipa (-):Gulu lofiirira lokha lokha limawonekera m'chigawo chowongolera (c). Palibe mabatani ofiira ofiira omwe adapezeka m'chigawo (T). Zotsatira zoyipa sizikuwonetsaClostridium Hardilematenda.

Zosavomerezeka:Palibe gulu lofiirira lofiirira m'magawo olamulira (c). Akuwonetsa kuwongolera kolakwika kapena kuwonongeka kwa mayeso. Pankhaniyi, werengani malangizowo mosamala ndipo samalankhulanso ndi mayeso atsopano. Ngati vuto

Kupitilira, muyenera kusiya nthawi yomweyo kugwiritsa ntchito nambala ya batch ndikulumikizana ndi wokondedwa wanu

Kusunga

1. 1. TheClostridium Hardile(Gdh) mayeso a antigen mwachangu ndi akatswiriin vitsroKugwiritsa ntchito matenda, ndipo kuyenera kugwiritsidwa ntchito kungodziwika kwa anthuClostridium Hardile.

2. 2. Zotsatira zake ziyenera kugwiritsidwa ntchito pokhapokha mutazindikira kuti ndi oleza mtima ndi zizindikiro ndi matendawa. Kuzindikira kwachipatala kuyenera kungopangidwa ndi dokotala pokhapokha atapeza ndalama zonse komanso zantchito zayesedwa.

3. 3. Monga momwe mungagwiritsire ntchito ma antibodies aliwonse ogwiritsa ntchito mbewa, kuthekera komwe kumachitika chifukwa chododometsa kwa munthu anti - ma antibodies (hama) m'chifanizo.

4. Mawu otchuka kuchokera kwa odwala omwe alandila ma antibododies a Monoclonies a matenda a matenda kapena chithandizo akhoza kukhala ndi hama. Zolinga zake zitha kuyambitsa zotsatira zabodza kapena zabodza.

4. 5. Monga mayeso onse ozindikira, kuzindikira kuyenera kungopangidwa ndi dokotala kokha pokhapokha atapeza zinthu zochipatala.
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