Kuyesa kwamphamvu

Kugwiritsa Ntchito

Mayeso a Lyme Bortalia IGG / IGM Kuyesa mwachangu ndi chromatographic yofulumira immunography kuti igg ndi ma antibodies a ma antibodies a borrelia. M'magazi a anthu, seramu kapena plasma.

Chiyambi

Matenda a Lyme, omwe amadziwikanso kuti Lyme Bortaliosis, ndi matenda opatsirana omwe amayambitsidwa ndi mabakiteriya a Borrelia. zomwe zimafalikira ndi nkhupakupa. Chizindikiro chodziwika bwino cha matenda ndi gawo lokulitsa lofiira pakhungu, lomwe limadziwika kuti erythema migrans, zomwe zimayamba pamalopo oluma pafupifupi sabata pambuyo pake. Pafupifupi 25 - 50% ya anthu omwe ali ndi kachilomboka samakulitsa zotupa. Zizindikiro zina zoyambirira zimatha kuphatikiza malungo, kupweteka mutu komanso kumva kutopa. Ngati sanasinthe, zingaphatikizepo kutaya mbali imodzi ya nkhope imodzi, kupweteka kwa mutu, kupweteka pakhosi ndi pambale, pakati pa ena. Miyezi mpaka patapita zaka, zigawo zobwerezabwereza zopweteka ndi zotupa zimatha kuchitika. Nthawi zina, anthu amakhala ndi ululu wowombera kapena kuwaza mikono ndi miyendo. Ngakhale chithandizo choyenera, pafupifupi 10 mpaka 20% ya anthu amakhala ndi zowawa zolumikizana, mavuto okumbukira, komanso amakhala otopa kwa miyezi isanu ndi umodzi.

Matenda a Lyme amafalikira kwa anthu poluma ndi nkhupakupa kwa gensus. Nthawi zambiri, yopeka iyenera kuphatikizidwa maola 36 mpaka 48 asanafalikire. Ku North America, Borrelia BurferorI ndi Bortalia Mayonii ndi zomwe zimayambitsa. Ku Europe ndi Asia, mabakiteriya Borrelia Affali ndi Bortalia Garinii amayambitsa matendawa. Matendawa samawoneka kuti akumasulidwa pakati pa anthu, ndi nyama zina, kapena chakudya. Kuzindikira kumakhazikika pa kuphatikiza kwa zizindikiro, mbiri ya kuwonetsedwa, ndipo mwina kuyesa ma antibodies apadera m'magazi. Kuyesedwa kwa magazi nthawi zambiri kumabweretsa mavuto m'masiku oyambirirawo. Kuyesedwa kwa nkhupakupa sikothandiza.Mhungu. ma antibodies mu magazi, seramu, kapena plasma.

Machitidwe

Lolani chida choyesa, fanizo, buffen, ndi / kapena kuwongolera kuti mufikire kutentha (15 30 ° C) musanayesedwe.

1. Bweretsani thumba ku kutentha kwa chipinda musanatsegule. Chotsani chida choyeserera kuchokera ku thumba losindikizidwa ndikugwiritsa ntchito mwachangu.
2. Ikani chida choyeserera pamalo oyera.

WaSeramu kapena plasma zonena: 

Gwirani dontho limodzi molunjika, jambulani fanizolimpakaLembani mzere (Pafupifupi 10 Ul), ndikusamutsa fanizoli ku chiwonetsero cha chipangizo choyeserera, kenako onjezerani madontho awiri a Buffer (pafupifupi 80 ml) ndikuyambitsa nthawi. Onani chithunzi pansipa. Pewani kutchera ma thovu a mpweya mu chinsalu (s).

WaMagazi athunthu (venyucunclence / chala) zonena:

Kugwiritsa ntchito dontho: gwiritsani dontho mokhazikika, jambulani fanizoli0,5 - cm 1 pamwambapa mzere, ndikusamutsa madontho awiri a magazi athunthu (pafupifupi 20 μl) kutsimikizika kwa chipangizochi, kenako onjezerani madontho awiri a buffer (pafupifupi 80 ul) ndikuyambitsa nthawi. Onani chithunzi pansipa.

Kuti mugwiritse ntchito micropipette: ma pipette ndi dial ya magazi athunthu 20 μl ya chipangizo choyeserera (onjezerani) a chipangizocho, kenako onjezerani madontho awiri (pafupifupi 80 μl) ndikuyambitsa nthawi.

3. Yembekezerani mzere wa utoto kuti uoneke. Werengani zotsatira za mphindi 10.Do osatanthauzira zotsatira pambuyo 20 mphindi.

Kutanthauzira kwa Zotsatira