Cryptosporidium antigen kuyesedwa mwachangu

Kugwiritsa Ntchito

Cryptosporidium parvam antigen mwachangu ndi chromatographraphic yofulumira imnospossim ya ku Cryptosporidium panduna wa bomba. Zotsatira zoyesedwa zimayenera kuthandiza pakuzindikira za Cryptosporidium parvam matenda ndikuwonetsetsa mphamvu ya achire chithandizo mankhwala.

Chiyambi

Cryptosporidiosis ndi matenda opatsirana omwe amayambitsidwa ndi Cryptosporidium parvum, yomwe nthawi zina imayambitsidwa ndi mitundu ina ya Cryptosporidium. Zizindikiro nthawi zambiri zimawoneka pafupifupi masiku 7 chifukwa cha matenda, kuphatikizapo kupweteka kwam'mimba, kutsekula m'mimba, kusanza ndi kutentha thupi. Zizindikiro za odwala ambiri mpaka masiku 6 mpaka 10, koma atha kwa milungu ingapo. Odwala Odwala Mavuto Athu a Mthupi, monga matenda, kungakhale kwakukulu komanso ngakhale pa moyo - kuwopseza Popeza lipoti la boma mu 1976, lapezeka kuti matendawa ali ponseponse, ndipo ndi tizilombo toyambitsa matenda tomwe timapezeka pa matenda obwera alendo. Pali odwala ambiri omwe ali ndi matenda ovuta ndi matendawa.

Njira Yoyeserera

Bweretsani ziyeso, zoyesazi, ndi / kapena kuwongolera kutentha kwa chipinda (15 - 30 ° C) musanagwiritse ntchito.

1. Chotsani mayesowo m'thumba lake losindikizidwa, ndikuyika pa oyera pamtunda. Lembani chipangizocho ndi chizindikiritso kapena chowongolera. Zotsatira zabwino kwambiri zomwe zikuyenera kuchitika mkati mwa ola limodzi.
2. Kukonzekera kwa Clumen

Omwe atulutsire botolo lachitsanzo, gwiritsani ntchito ogwiritsira ntchito omwe amaphatikizidwa nawo pa kapu kuti musinthe chidutswa chaching'ono (4 - 6 mm) Kwa madzi kapena semi - chopondera chokhazikika, onjezerani micreoliter 100 ya chopondapo ku vial ndi pipette yoyenera. Sinthani ndodo mu botolo ndikulimba. Sakanizani stool zitsanzo ndi buffer bwino pogwedeza botolo kwa masekondi angapo.

3. Njira Yanjira

3.1 Gwirani botolo lolunjika ndi mfundo yolowera kulowera kutali ndi wochita masewera olimbitsa thupi, ikani nsonga.

3.2. Gwirani botolo lokhazikika pa chiwonetsero cha khadi yoyeserera, ikani madontho atatu (120 μl) ya Stool Stool bwino ndikuyambira nthawi.

Pewani kutchera ma thovu a mpweya mu chinsalu (s), ndipo osawonjezera njira iliyonse yothandizira.

Pomwe mayeso amayamba kugwira ntchito, utoto umasamukira kudera lomwe lili pakatikati pa chipangizocho.

3.3. Yembekezerani gulu lazithunzi kuti muoneke. Werengani zotsatira pakati pa 5 - Mphindi 10. Chitsanzo chabwino champhamvu chitha kuwonetsa zotsatira zoyambirira.

Osatanthauzira zotsatira pambuyo 10 mphindi.

Pomwe mayeso amayamba kugwira ntchito, utoto umasamukira kudera lomwe lili pakatikati pa chipangizocho.

Kutanthauzira kwa Zotsatira

Zabwino: Awiri magulu achikuda amawonekera pa nembanemba. Bandi imodzi imawonekera kudera la Control (c) ndi gulu lina limapezeka m'chigawo (T).

Zoipa: gulu limodzi lokha lachida limapezeka m'chigawo chowongolera (c).Palibe gulu lachilengedwe lomwe limapezeka m'chigawo choyeserera (T).

Zosavomerezeka: Bandi lolamulira limalephera kuwonekera.Zotsatira za mayeso omwe sanatulutse bandi yowongolera nthawi yomwe yawerengedwa iyenera kutayidwa. Chonde onaninso njirayi ndikubwereza ndi mayeso atsopano. Ngati vutoli likupitilira, silimangogwiritsa ntchito zida nthawi yomweyo ndikulumikizana ndi wogulitsa kwanu.

1. Kuwongolera kwapadera

   • Zowongolera zamkati za procesdul zimaphatikizidwa poyesedwa. Gulu lauda lomwe limawonekera m'chigawo chowongolera (c) limawerengedwa njira yotsimikizika yamkati, ndikutsimikizira voliyumu yokwanira komanso yolondola yolondola.
   • Zowongolera zakunja siziperekedwa ndi izi. Ndikulimbikitsidwa kuti zowongolera zabwino komanso zoyipa zimayesedwa ngati njira yabwino yothandizira kutsimikizira njira yoyeserayo ndikutsimikizira kuyeserera koyenera.

Zoperewera za mayeso

1. Thecryptosporidium parvam antigen kuyeserera kwa Vigrocal ku matenda a Vitro ozindikira, ndipo ziyenera kugwiritsidwa ntchito pofufuza za Cryptosporidium parvam.
2. Zotsatira zoyesedwazo ziyenera kugwiritsidwa ntchito pokhapokha mutawunika ndi zizindikiro ndi zizindikiro za matendawa. Kuzindikira kwachipatala kuyenera kungopangidwa ndi dokotala pokhapokha atapeza ndalama zonse komanso zantchito zayesedwa.
3. Monga ndi lingaliro lililonse logwira ntchito sseji, kuthekera komwe kumachitika chifukwa chododometsa kwa munthu anti - ma antibodies (hama) m'chifanizo. Zofanana ndi odwala omwe alandila ma antibolodies a Monoclonies a matenda a matenda kapena chithandizo akhoza kukhala ndi hama. Zolinga zake zitha kuyambitsa zotsatira zabodza kapena zabodza.

• Monga mayeso onse ozindikira, kuzindikira kuyenera kungopangidwa ndi dokotala kokha pokhapokha atapeza zinthu zochipatala komanso zantchito zayesedwa.