Giardia fombo wa Antigen Firgen Flights

Kugwiritsa Ntchito

Giardia Forklia antigen Flightography ndi chromatographic yofulumira imnopassim imnossiy kupezeka kwa Giardass antigen m'nkhani yopanda kanthu. Zotsatira zoyesedwa zimayenera kuthandiza pakuzindikira za Giardia fombo wa nkhosa yamphongo ndikuwunika mphamvu ya mankhwala ochizira.

Zida

Zida zoperekedwa

- Zida zopendekera payokha

 Pakani phukusi

- Mapaipi otayika

 Machubu operewera ndi

Chotsani Buffer

Zida zofunika koma sizinaperekedwe

 Chidendeni Zowongolera

 Manja

Njira Yoyeserera

Bweretsani Ziyeso, Zoyesa, ndi / kapena Kuwongolera Kutentha Kwachipinda (15 - 30 ° C)musanagwiritse ntchito.

1. 1. Chotsani mayesowo m'thumba lake losindikizidwa, ndikuyika pa oyera pamtunda. Lembani chipangizocho ndi chizindikiritso kapena chowongolera. Zotsatira zabwino kwambiri zomwe zikuyenera kuchitika mkati mwa ola limodzi.

2. 2. Kukonzekera kwa centheni

Omwe atulutsire botolo lachitsanzo, gwiritsani ntchito ogwiritsira ntchito omwe amaphatikizidwa nawo pa kapu kuti musinthe chidutswa chaching'ono (4 - 6 mm) Kwa madzi kapena semi - chopondera chokhazikika, onjezerani micreoliter 100 ya chopondapo ku vial ndi pipette yoyenera. Sinthani ndodo mu botolo ndikulimba. Sakanizani stool zitsanzo ndi buffer bwino pogwedeza botolo kwa masekondi angapo.

3. 3.

3.1 Gwirani botolo lolunjika ndi mfundo yolowera kulowera kutali ndi wochita masewera olimbitsa thupi, ikani nsonga.

3.2. Gwirani botolo lokhazikika pa chiwonetsero cha khadi yoyeserera, ikani madontho atatu (120 μl) ya Stool Stool bwino ndikuyambira nthawi. Pewani kutchera ma thovu a mpweya mu chinsalu (s), ndipo osawonjezera njira iliyonse yothandizira. Pomwe mayeso amayamba kugwira ntchito, utoto umasamukira kudera lomwe lili pakatikati pa chipangizocho.

3.3. Yembekezerani gulu lazithunzi kuti muoneke. Werengani zotsatira pakati pa 5 - Mphindi 10. Chitsanzo chabwino champhamvu chitha kuwonetsa zotsatira zoyambirira.

Osatanthauzira zotsatira pambuyo 10 mphindi.

Pomwe mayeso amayamba kugwira ntchito, utoto umasamukira kudera lomwe lili pakatikati pa chipangizocho.

Kutanthauzira kwa Zotsatira

Zabwino: Magulu awiri achikuda amawonekera pa nembanemba.Bandi imodzi imawonekera kudera la Control (c) ndi gulu lina limapezeka m'chigawo (T).

Zoipa: gulu limodzi lokha lachida limapezeka m'chigawo chowongolera (c).Palibe gulu lachilengedwe lomwe limapezeka m'chigawo choyeserera (T).

Zosavomerezeka: Bandi lolamulira limalephera kuwonekera.Zotsatira za mayeso omwe sanatulutse bandi yowongolera nthawi yomwe yawerengedwa iyenera kutayidwa. Chonde onaninso njirayi ndikubwereza ndi mayeso atsopano. Ngati vutoli likupitilira, silimangogwiritsa ntchito zida nthawi yomweyo ndikulumikizana ndi wogulitsa kwanu.

ZINDIKIRANI:

1. Kukula kwa mitundu mu mayeso oyeserera (T) kumatha kukhala kosiyanasiyana malinga ndi kuchuluka kwa anthu wamba omwe ali pachithunzichi. Chifukwa chake, mthunzi uliwonse wa utoto m'chigawo choyeserera uyenera kuonedwa kuti ndi wabwino. Dziwani kuti iyi ndi mayeso oyenera pokhapokha, ndipo sangathe kudziwa kuchuluka kwa anthu wamba. Kugwiritsa ntchito voliyumu osakwanira, njira yolondola yogwiritsira ntchito kapena mayeso omaliza ndi zifukwa zambiri zopewera band band.

   1. Zoperewera za mayeso

   2. 1. Giardia amphonlia antigen mayeso a antigen ndi a katswiri mu kafukufuku mu matenda a Vitro, ndipo ayenera kugwiritsidwa ntchito pofufuza za khutu la anthuwatani.
   3. 2. Zotsatira zake ziyenera kugwiritsidwa ntchito pokhapokha mutazindikira kuti ndi oleza mtima ndi zizindikiro ndi matendawa. Kuzindikira kwachipatala kuyenera kungopangidwa ndi dokotala pokhapokha atapeza ndalama zonse komanso zantchito zayesedwa.
   4. 3. Monga momwe mungagwiritsire ntchito ma antibodies aliwonse ogwiritsa ntchito mbewa, kuthekera komwe kumachitika chifukwa chododometsa kwa munthu anti - ma antibodies (hama) m'chifanizo. Zofanana ndi odwala omwe alandila ma antibolodies a Monoclonies a matenda a matenda kapena chithandizo akhoza kukhala ndi hama. Zolinga zake zitha kuyambitsa zotsatira zabodza kapena zabodza.
   5. 4. Monga mayeso onse ozindikira, kuzindikira kuyenera kungopangidwa ndi dokotala kokha pokhapokha atapeza ndalama zonse zam'madzi zatha.
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