Clostridium Difficile Direfile Ikizamini cya Rapid

Gukoresha

Clostridium diffisile. Ibisubizo by'ibizamini byateguwe kugirango bifashe antibiyotike - bifitanye isano ni diarrhea colilitis na pseudomembranous colilitis iterwa no kwanduraClostridium diffisile.

Ihame

Gukoresha ihame rya Colloidal Zahabu Imyunochromatography, ihene anti - Imbeba Polyclon Antibody (urukurikirane c) nimbeba anti -Clostridium diffisileMonoclonal antibods yashyizwe muri filime ya selile. Imbeba monoclonal antibodies kuriC. BIFUMIzashyizwe ku masahani ya zahabu hamwe na colloidal zahabu. Iyo icyitegererezo cyiza kigeragezwa, theClostridium diffisileantigen murugero ruhuza imbebaClostridium diffisileMonoclonal antibody kuri padi ya zahabu, ikora uruganda rugenda runyura muri membrane binyuze muri chromatografiya. Nyuma yumurongo watahuye, yashinze uruganda rwa sandwich hamwe na antiboderi ya mbere yo guteza imbere amabara, kandi ihujwe n'ihene anti - Imbeba Ig Polyclon AntiBinds umurongo ushinzwe iterambere ryamabara, ibara ribi ryerekana gusa kumurongo mwiza.

Uburyo bw'ikizamini

Zana ibizamini, ingero, na / cyangwa kugenzura ubushyuhe bwicyumba (15 - 30 ° C)mbere yo gukoresha.

1. Kuraho ikizamini kuva umufuka wacyo ufunze, hanyuma ubishyire hejuru, urwego. Andika igikoresho gifite umurwayi cyangwa kugenzura indangamuntu. Ibisubizo byiza byasabwe mugihe kimwe.

2. Imyiteguro yerekana:

Kuramo cap of the FrimeUns Trube, hanyuma utabishaka wateje inkombe yicyitegererezo mubyiciro byibuze inshuro 3 zo gukusanya intebe (zihwanye na 1/4 cyamashaza). Fata imiyoboro ya kinini hamwe no gukuramo buffer ihagaritse, shyiramo inkoni yo gutoranya, ikanda hepfo yumuyoboro. Vanga stool spezen hamwe na buffer neza unyeganyeza icupa kumasegonda make.

3. Uburyo bwo Gukwirakwiza:

Kongera ingofero ku gukusanya amakuru, hanyuma uzunguze icyegeranyo cya kinini muguteza agaciro kugirango uvange urugero na buffer. Kureka umuyoboro wenyine muminota 2.

 Kuraho umupfundikizo muto hejuru.

 Fata icupa mumwanya uhagaze hejuru yicyitegererezo cyibikoresho byikizamini, tanga ibitonyanga 3 (hafi 90μl) byingoro yintebe ya didple (s) hanyuma utangire igihe.

Icyitonderwa:Irinde gufata nabi ikirere mubyiza (s) neza, kandi ntukongere igisubizo cyakarere kabisubizo.

Mugihe ikizamini gitangiye gukora, ibara rizamuka hirya no hino kubisubizo biri hagati yigikoresho.

 Tegereza itsinda ryamabara (s) kugaragara. Soma ibisubizo hagati ya 5 - iminota 10. Icyitegererezo cyiza gishobora kwerekana ibisubizo kare. Ntugasobanure ibisubizo nyuma yiminota 15. Mugihe ikizamini gitangiye gukora, ibara

kwimuka hirya no hino mu karere kari hagati yigikoresho.

Gusobanura ibisubizo

Ibyiza (+):Amababi abiri yumutuku aragaragara. Imwe iherereye mu karere katandurwa (T), ikindi giherereye mu karere keza (c).

Icyitonderwa:Itsinda ryijimye ritukura mukarere (T) birashobora kwerekana ibintu byijimye byijimye kandi byoroshye. Ariko, mugihe cyigihe cyihariye cyo kwitegereza, utitaye ku ibara ryitsinda, niyo bane bafite intege nke cyane

bigomba gusobanurwa nkibisubizo byiza.

Bibi (-):Gusa itsinda ryijimye rigaragara mukarere keza (c). Nta bande b'umutuku wabonetse mu karere kitandurwa (T). Ibisubizo bibi byerekana oyaClostridium diffisilekwandura.

Bitemewe:Nta banditsi b'umutuku mu karere keza (c). Yerekana imikorere cyangwa kwangirika kwikizamini. Muri uru rubanza, soma amabwiriza yongeye kwitonda kandi ugasubira hamwe nikigeragezo gishya. Niba ikibazo

ukomeje, ugomba guhita ureka gukoresha numero yicyiciro hanyuma ukavuga utanga isoko

Imbogamizi

1. 1.Clostridium diffisile(GDH) Ikizamini cya Rapid Rapid ni umwugain vitroGukoresha Gukoresha, kandi bigomba gukoreshwa gusa kubwo kumenya abantuClostridium diffisile.

2. 2. Ibisubizo byikizamini bigomba gukoreshwa gusa gusuzuma no kwihanganira ibimenyetso nibimenyetso byindwara. Isuzuma risobanutse rigomba gukorwa gusa na muganga nyuma yubuvuzi bwose bwa kavukire na laboratoire byasuzumwe.

3. 3. Nkibizamini byose bikoresha imbeba antibodies, ibishoboka bibaho kubabaza nabantu anti - Hama) murugero.

4. Ingero ziva kubarwayi babonye imyiteguro ya antibodies ya monoclonal yo gusuzuma cyangwa kuvura bishobora kuba birimo hama. Ibyingenzi birashobora gutera ibisubizo byiza cyangwa ibinyoma.

4. 5. Mugihe cyo kwipimisha gusa, isuzuma ryemejwe rigomba gukorwa gusa numuganga nyuma yubuvuzi bwose bwa kavukire nubutaka byasuzumwe.
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