Ikizamini cyihuse kandi cyizewe trypanomama kwa malariya p.f./pan antigen

Intangiriro

Malariya iterwa na protozoan itera selile itukura.1 Ishami ry'Umuryango w'Abibumbye ryisi rivuga ko miliyari 3,3 igereranya maliyari mu 2006, hamwe na miliyoni 247 z'izi miliyari zitezimbere (86% muri Afurika), hamwe na miliyoni 1 ( Ahanini abana b'Abanyafurika) bapfira indwara. Isesengura 2 Microscopic yanduye amaraso menshi kandi yoroheje yahinduwe ni tekinike isanzwe yo gusuzuma yo kumenya indwara za malariya mu kinyejana girenga. Tekinike ishoboye kwisuzumisha neza kandi yizewe mugihe ikozwe na microscopiste neza ukoresheje protocole yasobanuwe. Ubuhanga bwa microscopiste kandi bugakoresha muburyo bugaragara kandi busobanuwe, bikunze kwerekana inzitizi zikomeye zigera ku kugeraho byimazeyo ibishoboka byose bya microscopique. Nubwo hariho umutwaro wa lotistike ujyanye no gukora igihe - ubushishozi, umurimo - Ibikoresho Byinshi, kandi Ibikoresho Bisabwa ikoranabuhanga. Malariya P.FT igikoresho cyibizamini byipimisha (Amaraso yose) nikizamini cyihuse cyo kumenya ko P.Falle .vivax (p.v), p.ovale (p.o) na p.malariae (p.m). Ikizamini gikoresha zahabu ya Colloid ihuriweho no guhitamo P. Kumwihariko na Pan - P.f, P.V, p.O na P.M) mumaraso yose.

Ibikoresho

Ibikoresho byatanzwe

Ibikoresho birakenewe ariko ntibitangwa

Inzira

Emera igikoresho cyibizamini, ingero, buffer, na / cyangwa kugenzura kugirango ugabanye ubushyuhe bwicyumba (15 - 30 ° C) Mbere yo Kwipimisha.

Kuraho igikoresho cyibizamini uhereye kumufuka wa foil hanyuma uyikoreshe vuba bishoboka. Ibisubizo byiza bizaboneka niba isuzuma rikorwa mugihe cyisaha imwe.

Shira igikoresho cyibizamini kumurongo usukuye kandi uzinze. Kohereza icyitegererezo na pipette cyangwa umuyoboro:

Gukoresha pipette: Kwimura 10 mL y'amaraso yose kugirango abone neza - 1 (w1) yikikoresho cyibizamini, hanyuma ongeramo ibitonyanga 3 byuzuye bya buffer kugirango byiza - 2 (w2), hanyuma utangire igihe. (Reba urugero ① munsi). Irinde gufata umwambaro muri W1. Mu mpera yiminota 5, ongeraho 1 igitonyanga cyuzuye cya buffer kuri w1 (reba ishusho ③ munsi).

Gukoresha pipette igaragara: Fata umuyoboro uhagaritse; Shushanya umurongo ugana kumurongo uzuza nkuko bigaragara mu gishushanyo ① hepfo. Kohereza icyitegererezo kuri w1 yibikoresho byikizamini, hanyuma ongeraho ibitonyanga 3 byuzuye bya buffer kugeza W2 hanyuma utangire igihe. Irinde gufata umwambaro muri W1. Mu mpera yiminota 5, ongeraho 1 igitonyanga cyuzuye cya buffer kuri w1 (reba urugero ③ munsi).

3. Tegereza umurongo wamabara kugirango ugaragare. Ibisubizo bigomba gusomwa muminota 15. Ntukore

Sobanura ibisubizo nyuma yiminota 20.

Gusobanura ibisubizo

(Nyamuneka reba ikigereranyo kiri hejuru)

Ibyiza: * Imirongo ibiri cyangwa itatu itandukanye y'amabara agaragara.

1. Fallcirmum cyangwa Indwara ya Malariya:Umurongo umwe ugaragara mukarere kagenzura, umurongo umwe ugaragara mumurongo wa pan umurongo numurongo umwe ugaragara muri p.f Umurongo.
2. Indwara ya Faltict: umurongo umwe ugaragara mukarere kagenzura, kandi umurongo umwe ugaragara muri p.f.

Non - Amashanyarazi ya Falsmodium:Umurongo umwe ugaragara mukarere kagenzura kandi umurongo umwe ugaragara mumurongo wa pan umurongo.

.

Ibibi: Umurongo umwe gusa w'amabara ugaragara mukarere kagenzura.

Bitemewe: Umurongo wo kugenzura unaniwe kugaragara. Ubunini budahagije cyangwa tekinike idakwiye nuburyo bushoboka cyane bwo kugenzura umurongo. Ongera usuzume inzira hanyuma usubiremo ikizamini hamwe nigikoresho gishya cyikizamini. Niba ikibazo gikomeje, guhagarika ukoresheje ibikoresho bipima hanyuma ukavuga umuyoboke waho.

Kuri Pan: 99.20% (95% ci: 95.16%~> 99.99%)  

Kuri P.F:> 99.99% (95% CI: 93.02%~100.00%)

Ugereranije yihariye: 98.94% (95% ci:96.79%~99.79%) 

Ukuri: 99.15% (95% ci:97.76%~99.75%)