Rapid Escherichia Coli o157 ikizamini cyo kurwanya antigen - cyingenzi mubikoresho bya rotavirus

Escherichia Coli O157 Ikizamini cya Rapid Rapid ni Chromatografiya ku buryo bwihuse bwo kumenya Escherichia Coli O157 ihangayikishijwe na Stool ya muntu. Ibisubizo by'ibizamini byateguwe kugirango bifashe gusuzumwa impibondo

Escherichia Coli O157 Antigen Ikizamini cya Rapid

Ihame

Gukoresha ihame rya Colloidal Zahabu Imyunochromatography, ihene anti - Imbeba Polyclonal antibody (urukurikirane c) nimbeba anti - Escheichia Coli O157 Monoclonal AntiBers muri Film. Imbeba Monoclonal Antibolies kuri Escherichia Coli O157 yakosowe ku masahani ya zahabu hamwe na labels za zahabu za colloidal. Iyo icyitegererezo cyiza kigeragezwa, Theescheichia Coli O157 Antigen murugero ruhuza imbeba kuri pausecheichiali o157 monocLacheichialing kuri zahabu, igakora ingero zigenda zinyuze muri chromatografiya. Nyuma yumurongo watahuye, yashinze uruganda rwa sandwich hamwe na antiboderi ya mbere yo guteza imbere amabara, kandi ihujwe n'ihene anti - Imbeba Ig Polyclon AntiBinds umurongo ushinzwe iterambere ryamabara, ibara ribi ryerekana gusa kumurongo mwiza.

Ugereranije na sessicutivite: 96.15% (95% Ci: 91.25% ~ 98.74%)

Ugereranije yihariye: 98.75% (95% Ci: 96.84% ~ 99,66%)

Ukuri: 98.00% (95% Ci: 96.25% ~ 99.08%)

Ibice

Uburyo bw'ikizamini

Zana ibizamini, ingero, na / cyangwa kugenzura ubushyuhe bwicyumba (15 - 30 ° C) mbere yo gukoresha.

1.Remove ikizamini kuva umufuka wacyo ufunze, hanyuma ubishyire hejuru, urwego. Andika igikoresho gifite umurwayi cyangwa kugenzura indangamuntu. Ibisubizo byiza byasabwe mugihe kimwe.

2.Gutegura imyiteguro:

Kuramo Cap of the Curtimen gukusanya, hanyuma utabishaka wateje inkombe yicyitegererezo mubyiciro byindangamitsi byibuze inshuro 3 zo gukusanya hafi umwanda (bihwanye na 1/4 cyamashaza). Fata imiyoboro ya kinini hamwe no gukuramo buffer ihagaritse, shyiramo inkoni yo gutoranya, ikanda hepfo yumuyoboro. Vanga stool spezen hamwe na buffer neza unyeganyeza icupa kumasegonda make.

1.Ibitekerezo byinshi:

• Kongera ingofero kuri tube yo gukusanya urugero, hanyuma uzunguze icyegeranyo cya kinini tube imbaraga zo kuvanga icyitegererezo na buffer. Kureka umuyoboro wenyine muminota 2.
• Kuraho umupfundikizo muto hejuru
• Fata icupa mumwanya uhagaze hejuru yicyitegererezo cyikarita yikizamini, tanga ibitonyanga 3 bigera kuri 90μl) byingoro nziza kuri sample (s) hanyuma utangire igihe.

Icyitonderwa:Irinde gufata nabi ikirere mumitsi (s) neza, kandi ntukongere igisubizo cyabisubizo.

Mugihe ikizamini gitangiye gukora, ibara rizamuka hirya no hino kubisubizo biri hagati yikikoresho.

• Tegereza itsinda ryamabara kugirango ugaragare. Soma ibisubizo hagati ya 5 - iminota 10. Icyitegererezo cyiza gishobora kwerekana ibisubizo kare. Ntugasobanure ibisubizo nyuma yiminota 15. Mugihe ikizamini gitangiye gukora, ibara rizamuka hirya no hino kubisubizo biri hagati yikikoresho.

Gusobanura ibisubizo

• Ibyiza (+):Amababi abiri yumutuku aragaragara. Imwe iherereye ahantu hatanyamye (T), undi iherereye ahantu heza (c)

Icyitonderwa:Itsinda ryijimye ritukura muburyo bwo gutahura (t) rishobora kwerekana ibintu byijimye byijimye kandi byoroshye. Ariko, mugihe cyigihe cyagenwe, tutitaye ku ibara ry'itsinda, ndetse n'amatsinda adakomeye cyane agomba gusobanurwa nkibisubizo byiza.

• Bibi (-):Gusa itsinda ryijimye rigaragara muburyo bwo kugenzura ubuziranenge (c). Nta bande b'umutuku wabonetse ahantu hatanyamo (T). Igisubizo kibi cyerekana noescherichia coli o157 kwandura.
• Bitemewe:• Bitemewe: Nta banditsi b'umutuku mu gace keza (c). Yerekana imikorere cyangwa kwangirika kwikizamini. Muri uru rubanza, soma amabwiriza yongeye kwitonda kandi ugasubira hamwe nikigeragezo gishya. Niba ikibazo gikomeje, ugomba guhita ureka gukoresha numero yicyiciro hanyuma ukavuga utanga isoko.
• Intangiriro yimari

Hangzhou Umupfumu Cotech Co., Ltd numuryango wambere mumatsinda ya Immwungo. Ikipe ya Hangzhou ibi bioteki byateye uruhererekane rwa poroteyine n'ibikoresho byibizamini byihuse kubikoresho bya vitro bisuzumwe bya vitro. Buhoro buhoro, Imyuno yari azwi neza nka mugenzi wawe mwiza r & d kandi utanga umusaruro mwiza wibicuruzwa byipimisha. Hamwe no kwihangana cyane no gushora imari mugushushanya no guteza imbere ivd ugereranije nibikoresho byikizamini, twabonye ibintu byinshi bitera inkunga mumwaka ushize, cyane cyane mumwanya wo gusuzugura.