Igishushanyo gishobora kongerwa kubizamini bya leta byihuse - SARS - COV - 2 Kutabogama antibody Ikizamini cya Rapid - Imyuno

Gukoresha

Ikizamini cyihuse cyo kumenya icyiciro cya antibodiyite kumwanya - Cov - Inkingo zazo mumaraso yose, Serumu, cyangwa plasma.

Kubimwuga mugukoresha vitro gusa.

Byemewe impamyabumenyi

1. GE CEMEWE

2. Urutonde rwera rwamaguka

Ibiranga

A. Kwipimisha amaraso, amaraso y'urutoki rwose arakorwa.

B. CutOff ni 50ng / ml

C. Biroroshye gukora, nta bikoresho byiyongera byasabwaga gukora isuzuma

D. Icyitegererezo gito kirasabwa. 10Ubukorikori, Plasma cyangwa 20Ul yamaraso yose arahagije.

IkizaminiProcelit

Emera igikoresho cyibizamini, ingero, buffer, na / cyangwa kugenzura kugirango ugabanye ubushyuhe bwicyumba (15 - 30 ° C) Mbere yo Kwipimisha.

1) Zana umufuka kugeza ubushyuhe bwicyumba mbere yo gufungura. Kuraho igikoresho cyibizamini kuva umufuka ufunze hanyuma uyikoreshe vuba bishoboka.

2) Shira igikoresho cyibizamini kumurongo usukuye kandi utambitse.

Kuri Serumu cyangwa Plasma Ingero:

Fata igitonyanga gihagaritse, shushanya umurongo ugana kumurongo wuzuza (hafi 10 μl), hanyuma wohereze urugero rwibikoresho byipimisha, hanyuma ongeraho ibitonyanga 3 bya buffer (hafi 120 ml) hanyuma utangire igihe . Reba urugero hepfo. Irinde gufata nabi ikirere mumitsi (s).

Kumaraso yose (snipuncture / urutoki) ingero:

Gukoresha igitonyanga: Fata igitonyanga gihagaritse, shushanya umurongo 0.5 - cm 1 hejuru yumurongo wuzuza, hanyuma wimure ibitonyanga 2 byamaraso yose (s ongeraho ibitonyanga 2 ya buffer (hafi 90 UL) hanyuma utangire igihe. Reba urugero hepfo.

Kugira ngo ukoreshe micropipette: Pipet na Impaka 20 μl y'amaraso yose kugeza ku kigero cy'ibikoresho by'ibizamini, hanyuma ongeraho ibitonyanga 3 bya buffer (hafi 120 μl) hanyuma utangire igihe. Reba urugero hepfo.

3) Tegereza umurongo wamabara kugirango ugaragare. Soma ibisubizo muminota 10. Ntugasobanure ibisubizo nyuma yiminota 15.

- Byiza (+): Gusa c umurongo ugaragara, cyangwa t umurongo uhwanye na c umurongo cyangwa intege nke kurenza umurongo. Byerekana ko hari ibirango - Cov - 2 Antibodiyiriye antibodiyiriye murugero.

- Bibi (-): Umurongo wa T hamwe na C na C Ugaragara, mugihe ubukana bwa TUR bukomeye kuruta umurongo wa C. Byerekana ko nta mwanda uhari - Consury Antibodiyisiti ya 2 itendukira kuri urugero, cyangwa ikindi cyindirimbo ya SARS - COV - Gutesha agaciro Antibodi yabantuho kurwego rwo hasi cyane.

- Bitemewe: Umurongo wo kugenzura wananiwe kugaragara. Ingano idahagije cyangwa tekinike yuburyo butariyo nibyinshi