West Nili Virus Antigen Ikizamini cya Rapid

Ihame

West Nili virusi ya Antigen Ikizamini cya Rapid ni sandwich Phase Pumbemromatograchic. Gukora ikizamini, aliquot yicyitegererezo cyongewe kuri sample iriba ryibizamini byikizamini. Icyitegererezo kinyura muri padi kirimo antibodies kurwanya iburengerazuba virusi ya Nili farusi igihuze - Zahabu ya Collowal. Niba icyitegererezo kirimo Ubushingerazuba bwa Nili, Antigen azahuza na antibody yahitanye ibice bya zahabu ya colloidal kugirango agire antigen - antibody - Ibigo bya zahabu. Ibi bikago byimuka kuri membranesllsellse hamwe nigikorwa cya capillary yerekeza mukarere kigeragezwa hagati yububiko bwa virusi yububiko bwa nili Nkuko bigoye kugera kumurongo wikizamini, bazahambira bantibody bahuye na bagiteri kumutwe kugirango bibe umurongo. Umurongo utukura uzahora ugaragara mubisubizo kugirango werekane ko ikizamini cyakozwe neza nigikoresho cyibizamini neza. Niba bagiteri idahari cyangwa iri munsi yumupaka watangirwa, gusa umurongo wo kugenzura uzagaragara. Niba umurongo wo kugenzura utarwaye, ikizamini nticyemewe.

Ibikoresho

Ibikoresho byatanzwe

Uburyo bw'ikizamini

Emera ikizamini, reagents, swab strimen, na / cyangwa kugenzura kugirango ugere ku bushyuhe bwicyumba (15 - 30 ° C) Mbere yo Kwipimisha.

1. Kuraho cassette yikizamini uhereye kumufuka wa foo hanyuma uyikoreshe mugihe cyisaha imwe. Ibisubizo byiza bizaboneka niba ikizamini gikorewe ako kanya nyuma yo gufungura umufuka wa Foil.
2. Gukuramo virusi ya Nili Nili antigero ukurikije ubwoko bwimbitse.

Gusobanura ibisubizo

Ibyiza: Amababi abiri y'amabara agaragara kuri membrane.Itsinda rimwe rigaragara mukarere kagenzura (C) hamwe nindi tsinda rigaragara mukarere kizamini (T).

Ibyingenzi: Gusa itsinda rimwe ryamabara rigaragara mukarere kagenzura (C).Nta murongo ugaragara ibara rigaragara mukarere kizamini (T).

Bitemewe: kugenzura itsinda ryananiwe kugaragara.Ibisubizo bivuye mubizamini byose bitabyaye itsinda ryagenwe mugihe cyagenwe kigomba gutabwa. Nyamuneka suzuma inzira hanyuma usubiremo nikizamini gishya. Niba ikibazo gikomeje, guhagarika ukoresheje ibikoresho ako kanya hanyuma uhamagare umugaba wawe.

Icyitonderwa:

1. Ubukana bwamabara mukarere kakiza (T) birashobora gutandukana bitewe no kwibanda kubisesengura bitabiriye urugero. Kubwibyo, igicucu cyose cyamabara mugice cyikizamini kigomba gufatwa nkicyiza. Menya ko iyi ari ikizamini cyujuje ubuziranenge gusa, kandi ntishobora kumenya kwibanda ku gusesengura mu kigero.
2. Ingano idahagije, uburyo bwo gukora nabi cyangwa ibizamini byarangiye nuburyo bushoboka cyane bwo kugenzura ibipimo bya bande.

Imipaka yikizamini

1. Ikizamini cya Nili Nili cyo mu burengerazuba kirwanya umwuga mukoresha vitro gusuzuma, kandi kigomba gukoreshwa gusa ku buryo bujuje ubuziranenge bwa virusi ya Nili.
2. Igisubizo cyibizamini kigomba gukoreshwa kugirango usuzume kwihangana nibimenyetso nibimenyetso byindwara. Isuzuma risobanutse rigomba gukorwa gusa na muganga nyuma yubuvuzi bwose bwa kavukire na laboratoire byasuzumwe.
3. Kimwe nigipimo icyo ari cyo cyose gikoresha imbeba antibodies, ibishoboka bibaho kuba indaho ku bantu kurwanya abantu anti - Hama) mu kigero. Ingendo zituruka ku barwayi babonye imyiteguro ya antibodis ya monoclonal yo kwisuzumisha cyangwa kuvura bishobora kuba birimo hama. Ibyingenzi birashobora gutera ibisubizo byiza cyangwa ibinyoma.
4. Mugihe ibizamini byose byo gusuzuma, isuzuma ryemejwe rigomba gukorwa gusa numuganga nyuma yubuvuzi bwose bwa kavukire nubutaka byasuzumwe.

Ukuri: 96.27% (95% CI: 93.03%~98.28%)