Chikungunya Igg / Igm Rapid bvunzo

Yakatarisirwa kushandiswa

The Chikungunya Igg / Igm Phiage bvunzo ndeyekupedzisira kuyerera chromatopas nekuda kweiyo Quitative Kuwanikwa kweGg / Igm Anti Iyo inoitirwa kushandiswa seyedzo yekuongorora bvunzo uye seyamubatsiri mukuongororwa kwehutachiona neChik. Chero ipi neipi fungidziro ine Chikungunya Igg / IGM nekukurumidza bvunzo inofanira kusimbiswa neimwe nzira yekuyedza (s) uye kiriniki yakawanikwa.

Zvigadzirwa

Zvigadzirwa zvakapihwa

 Yemamwe yakarongedzwa bvunzo dzekuyedza

 Assay Buffer

 kuraswa kufungidzira kudonhedza

 Package isa

Zvigadzirwa zvinodiwa asi zvisina kupihwa

 fungidziro yekuunganidza mudziyo

 lancet

 Centrifuge

 Timer

Kuongorora maitiro

Bvumira bvunzo, reagents, swab fungidziro, uye / kana kudzora kusvika kukamuriKupisa (15 - 30 ° C) usati waedza.

1.Bring iyo pouch yekuisa tembiricha usati wavhuka. Bvisa mudziyo wekuedzwa kubva muhomwe yakavanzika uye uishandise nekukurumidza sezvinobvira.

2.Place mudziyo wekuyedza pane yakachena uye yakajeka nzvimbo.

Kune serum kana plasma fungidzira:

Bata dressed vertically, dhirowa iyo fungidziro kusvika kune inozadzwa mutsara (anenge 5 μl), uye chinja fungidziro kune iyo yechiitiko chebvunzo, wozowedzera 3 madonhwe eAsyy buffer (anenge 90 l)

uye tanga iyo timer. Dzivisa kuteedzera mhepo mabhuru mune iyo fungidziro tsime (s).

Yeropa rose (Venipuncture / zvigunwe zveminwe) fungidziro:

• Kuti ushandise dress: Dhizaina madonhwe, 0.5 - 1 cm pamusoro peiyo mutsara wetsetse, uye kufambisa kweropa rose (s) kune iyo bvunzo yeruzivo, wozowedzera 3 madonhwe 3 madonhwe of Assay buffer (anenge 90 μL) uye kutanga iyo timer.
• Kuti ushandise micropipeette: pipette uye kuparadzira eropa rese kune iyo fungidziro tsime (s) yeyekuedzwa mudziyo, wozowedzera 3 madonhwe eBuffer (anenge 90 μl) uye kutanga nguva.

3.WAIT yeiyo mutsara wemavara (s) kuoneka. Verenga mhinduro pamaminitsi gumi. Usadudzira mhedzisiro mushure memaminitsi makumi maviri.

Kududzira kwemhedzisiro

Igg yakanaka: *Purplish mutsvuku mutsara unoonekwa mune yekudzora mutsara wedunhu (c), uye chirevo chitsvuku chetsvina chinoonekwa muyedzo mutsara wedunhu (g). Mhedzisiro yacho yakanaka kwazvo yeChungununya yakanangana - ingangove inoratidza yechipiri Chukugugunya utachiona.

Igm Kubatsira: *Chiratidzo chitsvuku chinoonekwa mune yekudzora mutsara wedunhu (c), uye chirevo chitsvuku chakatsvuka chinoonekwa muyedzo mutsara wedunhu (m). Mhedzisiro yacho yakanaka kwazvo yeChikugunya yakanangana - IGM antibodies uye iri chiratidzo cheChirungu Chikunya utachiona.

Igg uye igm yakanaka: *Purlish mutsvuku mutsara unoonekwa mune yekudzora mutsara wedunhu (c), uye maviri ePurish matsvuku mitsara inofanirwa kuoneka muyedzo mutsara wedunhu g uye m, uye ruvara rwemavara emitsara haafanire kuenderana. Kukonzeresa Igg uye Igm Antibodies aive akanaka, achiratidza kuvapo kweAnti - Chik Igg uye Igm mumhando.

* Ongorora:Naizvozvo, chero mumvuri weruvara muyedzo mutsara wedunhu (s) (g uye / kana m) inofanira kunzi yakanaka.

Zvisina kunaka:Purlish mutsvuku mutsara unoonekwa mune yekudzora mutsara wedunhu (c). Hapana mitsara inoonekwa muyedzo mutsara wedunhu g uye m.

NVILID:Hapana mutsara unoonekwa pane mutsara wekudzora (c). Isina kukwana sampling huwandu kana zvisina kukodzera sampling nzira inogona kutungamira kune mhosva yekudzora mutsara.

Tarisa iyo yekuwedzera maitiro uye dzokorora maitiro ane iyo itsva bvunzo mudziyo. Kana dambudziko rikaramba riripo, imbomira kushandisa bvunzo suite pakarepo uye bvunza wako kugoverwa.

Kusimudzwa

1. 1. Maitiro ekuedzwa uye kududzirwa kwemhedzisiro kunofanirwa kutevedzerwa zvakanyanya kana kuongorora kuvapo kweGM ALM anti - Chik muSerum, plasma kana ropa kana ropa rose kubva pane imwe nhoroondo. Kutadza kutevera iyo

Maitiro anogona kupa mhinduro isiriyo.

2. 2. Kusimba kwenzvimbo yeyedzo haina mutsara wekuenderana ne

Antibody titter mune fungidziro.

3. 3. Mhedzisiro isina kunaka yechinyorwa chomunhu chinoratidza kusavapo kweshoko igm anti - Chik. Nekudaro, mhedzisiro yakaipa yebvunzo haina kudzikisira mukana wekuratidzwa kana kutapukirwa neChik.

4. 4

Sampuli inounganidzwa.

5. 5. Mamwe mafungidziro ane zvinhu zvisina kujairika zvegumi zvekutamba kwehutachiona hwehutachiona kana rheumatoid chinhu chinogona kukanganisa mhedzisiro inotarisirwa.

6. 6. Mhedzisiro yakawanikwa nemubvunzo uyu inofanira kungodudzirwa pamwe chete nedzimwe nzira dzekuongorora uye nezvakarukwa.
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