Cryptoporidium antigen nekukurumidza bvunzo

Yakatarisirwa kushandiswa

Cryptosporridium Parvum Antigen nekukurumidza bvunzo ndeyekupi kweRROMatoPasic Upunassay yekuwanikwa kweCryptoporidium Parvum Antigen muStool Stool Stool Sport. Mhedzisiro yebvunzo inoitirwa kubatsira mukuongororwa kweCryptoporidium Parvum utachiona uye kuongorora kushanda kwekurapa kwekurapa.

Nhanganyaya

Cryptossporosidois chirwere chinotapukira chakakonzerwa neCryptoporidium Parvum, iyo dzimwe nguva inokonzerwa nemamwe marudzi eCryptoporidium. Zviratidzo zvinowanzoonekwa zvinenge mazuva manomwe mushure mekutapukirwa, kusanganisira kurwadziwa mudumbu, marara emvura, kurutsa nefivha. Zviratidzo zvevarwere vazhinji vekupedzisira mazuva matanhatu kusvika gumi, asi vanogona kugara kwevhiki dzinoverengeka. Varwere vane matambudziko emidziyo, senge hutachiona, vanogona kuve vakanyanya uye kunyange hupenyu - kutyisidzira. Sezvo iyo hurukuro yepamutemo muna 1976, yakawanikwa kuti chirwere ichi chakapararira, uye chakajairika pathogen cherudzi rwevashanyi. Kune varwere vazhinji neAIDS yakaoma nechirwere.

Kuongorora maitiro

Huya nemiedzo, fungidziro, uye / kana kudzora kumhepo tembiricha (15 - 30 ° C) usati washandiswa.

1. Bvisa muedzo kubva muhomwe yayo yakavharwa, uye uiise pane yakachena, nhanho nzvimbo. Raira chishandiso chine murwere kana kudzora kuzivikanwa. Kuti uwane mhinduro dzakanakisa iyo leaday inofanira kuitwa mukati meawa imwe.
2. Fungidziro yekugadzirira

Unsungisa bhodhoro rekusimbisa, shandisa iyo yakasungirirwa tsvimbo yakabatanidzwa pane iyo cap yekuendesa chidiki chidimbu cheStool (4 - 6 mm muDhivha - mazana maviri mg - mazana maviri emamita Zvemvura kana semi - zvimbudzi zvakasimba, wedzera zana microcders ezvikanda kune vial ne pipette yakakodzera. Tsiva tsvimbo mubhodhoro uye simbisa zvakachengeteka. Sanganisa STOL Sampuli neBuffer zvakakwana nekuzunza bhodhoro remasekondi mashoma.

3. Assay maitiro

3.1 Bata bhodhoro remuenzaniso rakatwasuka nenjanji inonongedza kutungamira kure neyebvunzo mutambi, bvisa bukira.

3.2. Bata bhodhoro munzvimbo yemitambo pamusoro peiyo sampuli yakanaka yekadhi bvunzo, kuendesa 3 madonhwe (120 - 150 μl) yeiyo yakadzvanywa stool sampuli mushe (s) uye kutanga iyo timer.

Regedza kuteedzera mhepo mabhuru mumifananidzo mufungidziro zvakanaka (s), uye usawedzera chero mhinduro kune iyo mhedzisiro.

Sezvo bvunzo inotanga kushanda, ruvara ruchatamira pane iyo mhedzisiro yenzvimbo pakati pechishandiso.

3.3. Mirira bhendi reruvara (s) kuti rionekwe. Verenga mhedzisiro pakati pe5 - Maminitsi gumi. Muenzaniso wakasimba wakasimba ungaratidzwa.

Usadudzira mhedzisiro mushure memaminitsi gumi.

Sezvo bvunzo inotanga kushanda, ruvara ruchatamira pane iyo mhedzisiro yenzvimbo pakati pechishandiso.

Kududzira kwemhedzisiro

Zvakanaka: Piri mabhendi ane mavara anoonekwa pane membrane. Rimwe bhendi rinoonekwa munzvimbo yekutonga (c) uye imwe bhendi inoonekwa munzvimbo yeyedzo (t).

Zvisina kunaka: imwe chete yebhendi chete inoratidzwa munzvimbo yekutonga (c).Hapana chinooneka band bhendi chinoonekwa munzvimbo yeyedzo (t).

Isingabvumirwe: Bhendi rekutonga rinotadza kuoneka.Mhedzisiro kubva kune chero bvunzo iyo isina kuburitsa bhendi yekudzora panguva yakatarwa yekuverenga inofanira kuraswa. Ndokumbirawo uongorore maitiro uye dzokorora nemubvunzo mutsva. Kana dambudziko rikaramba riripo, rikarega kushandisa kit ipapo uye bvunza wako kugoverwa.

1. Mhando yekudzora

   • Yemukati maitiro ekutonga inosanganisirwa muedzo. Bhendi reruvara reruvara rinoonekwa munzvimbo yekutonga (c) inoonekwa seyakajeka maitiro ekutonga, kusimbisa kukwana kufungidzira vhoriyamu uye kururamisa maitiro maitiro.
   • Kunze kutonga hakufanirwe kupihwa neiyi kit. Zvinokurudzirwa kuti zvinhu zvakanaka uye zvisina kunaka zvinoedzwa sechiitiko chakanaka chekurambidza kusimbisa maitiro ebvunzo uye kuratidza maitiro akakodzera ekuedzwa.

Kugumira kwebvunzo

1. Thecryptopororidium Parvum Antigen Philid bvunzo ndeye nyanzvi muVitro kuongorora, uye inofanirwa kushandiswa chete pakuonekwa kwekuchema kwevanhu cryptoodoum parvum.
2. Mhedzisiro yebvunzo inofanirwa kushandiswa chete kuongorora nemurwere nezviratidzo nezviratidzo zvechirwere. Kuongororwa kwekiriniki kunokosha chete kunofanirwa kuitwa nachiremba mushure mekunge zvese zvekiriniki uye zvekutambisa zvakaongororwa.
3. Sezvinei nechero munhu asingaoneki zvekushandisa mbeva, mukana uripo wekuvhiringidza munhu anti - Mouse Antibodies (Hama) mune fungidziro. Ongdes kubva kune varwere vakagamuchira gadziriro yeMonownal antibodies ekuongororwa kana kurapa kunogona kunge kune hama. Mhedzisiro dzakadaro dzinogona kukonzera nhema dzakanaka kana dzenhema dzakaipa mhinduro.

• Sezvo zvese zviongorori zvekuongorora, kuongororwa kwakasimbiswa kunofanirwa kuitwa nachiremba mushure mekunge zvese zvechiremba uye zviratidzwe zvakaongororwa.