Rotavirus antigen nekukurumidza bvunzo

Yakatarisirwa kushandiswa

Rotaviru Antigen nekukurumidza bvunzo ndeyekukurumidza kweChromatographicic impunassay yekuwanikwa kwekuwanikwa kwehunhu hweRotavirus antigen mune vanhu stool stool. Mhedzisiro yebvunzo inoitirwa kubatsira mukuongororwa kwehutachiona hweRotavirus uye kuongorora kushanda kwekurapa kwekurapa.

Pfupiso

Infantile Diarrhea iboka rezvimwe zvinhu zvePediatric Zvirwere zvinokonzerwa nezvikomo zvakawanda uye zvinhu, zvinoratidzwa nekuwedzera kuwanda uye shanduko muzvimiro zvechitsiko. 80% yemanyowa emuchina anokonzerwa nehutachiona. Iyo huru pathogen yehutachiona enteritis ndeye Rotavirus, ichiteverwa neEnterovirus, yakadai seynestinavirus.

Rotavirus ndeimwe yematanho makuru anokonzeresa infantile manyoka. Iyo inonyanya kurwara zvidiki zvemukati epithelial masero, zvichikonzera kukuvara cell uye manyoka. Rotavirus yakapararira muzhizha, autumn uye chando gore rega rega. Iyo nzira yehutachiona ndeye fecal muromo nzira. Kuratidzwa kwekiriniki kuri kukanganisa gastroenteritis uye Osmotic Riya.

BvunzoMaitiro

Huya nemiedzo, fungidziro, uye / kana kudzora kumhepo tembiricha (15 - 30 ° C) usati washandiswa.

1. Bvisa muedzo kubva muhomwe yayo yakavharwa, uye uiise pane yakachena, nhanho nzvimbo. Raira chishandiso chine murwere kana kudzora kuzivikanwa. Kuti uwane mhinduro dzakanakisa iyo leaday inofanira kuitwa mukati meawa imwe.
2. Fungidziro yekugadzirira

Unsungisa bhodhoro rekuratidzira, shandisa iyo yakasungirirwa simple yakasungirirwa pane iyo cap yekuendesa chidiki chidiki cheStool (4 - 6 mm mune diameter; inenge makumi mashanu mg - 200 mg) muAsyy Buffer Tube. Zvemvura kana semi - zvimbudzi zvakasimba, wedzera zana microcders ezvikanda kune vial ne pipette yakakodzera. Tsiva tsvimbo mubhodhoro uye simbisa zvakachengeteka. Sanganisa STOL Sampuli neBuffer zvakakwana nekuzunza bhodhoro remasekondi mashoma.

3. Assay maitiro

3.1 Bata leary buffer tube yakatwasuka neyekukomba yakananga kune iyo nzira kure neye bvunzo Mutambi, bvisa bundu.

3.2. Bata bhodhoro munzvimbo yemitambo pamusoro peiyo sampuli yakanaka yekadhi bvunzo, kuendesa 3 madonhwe (120 - 150 μl) yeiyo yakadzvanywa stool sampuli mushe (s) uye kutanga iyo timer.

Regedza kuteedzera mhepo mabhuru mumifananidzo mufungidziro zvakanaka (s), uye usawedzera chero mhinduro kune iyo mhedzisiro.

Sezvo bvunzo inotanga kushanda, ruvara ruchatamira pane iyo mhedzisiro yenzvimbo pakati pechishandiso.

3.3. Mirira bhendi reruvara (s) kuti rionekwe. Verenga mhedzisiro pakati pe5 - Maminitsi gumi. Muenzaniso wakasimba wakasimba ungaratidzwa.

Usadudzira mhedzisiro mushure memaminitsi gumi.

Sezvo bvunzo inotanga kushanda, ruvara ruchatamira pane iyo mhedzisiro yenzvimbo pakati pechishandiso.

Kududzira kwemhedzisiro

Zvakanaka: Pirikana vatatumabhendi ane mavara anoonekwa pane membrane. Rimwe bhendi rinoonekwa munzvimbo yekutonga (c) uye imwe bhendi inoonekwa munzvimbo yeyedzo (t).

Zvisina kunaka: imwe chete yebhendi chete inoratidzwa munzvimbo yekutonga (c).Hapana chinooneka band bhendi inoonekwa munzvimbo yeyedzo (t).

Isingabvumirwe: Bhendi rekutonga rinotadza kuoneka.Mhedzisiro kubva kune chero bvunzo iyo isina kuburitsa bhendi yekudzora panguva yakatarwa yekuverenga inofanira kuraswa. Ndokumbirawo uongorore maitiro uye dzokorora nemubvunzo mutsva. Kana dambudziko rikaramba riripo, rikarega kushandisa kit ipapo uye bvunza wako kugoverwa.