Qalliinka 'Clouttridium' gdh, toxin a iyo toxin b combo mempid mempbot

Adeegsiga loogu talagalay

Sumtridium-ka 'Extridium-ka GDH, Toxin A iyo Toxin B Com Combo Combod Tijaabada ah ee Combo Congbodrenase iyo Toxins Antigen, GlouydrogeneNen, sida shaashad ka ah joogitaanka C. Joogsiga , waxayna xaqiijinaysaa jiritaanka sunta toxigenic C. Difiqida iyadoo la ogaanayo sunta a iyo b ee saxarada muunadaha laga qabo dadka looga shakisan yahay inay qabaan C. Imtixaanku wuxuu u adeegi doonaa sida ku-xisaabtanka ah ee ku saabsan ogaanshaha qaabdhismeedka ku-xigta. Sida kale ee C. D. Defformit Imtixaannada, natiijooyinka waa in loo qaddariyo iyadoo lala kaashanayo taariikhda caafimaad ee bukaanka.

Kooban

Clombtridium diffflinile waa bakteeriyada anaerobic-ka ah ee u shaqeysa cudurka loo yaqaan 'stategrity' oo ay tahay cudurka loo yaqaan 'stategrity' oo ah cudurka loo yaqaan 'cudurka' cudurka loo yaqaan 'pathestiture'^1,2,3 Xadhiga toxinogenic ee isku xidhka 'Clomtridium' Clombridium Chility waxay keentaa infekshanka infronk - shuban ilaa PseudomemberMbranous Colitis Coast, oo suurta gal ah in la dhinto. Cudur waxaa sababa laba sunta ah oo ay soo saareen xargaha sunta toxinogenic ee c.diftile: toxin a (madaafiicda ah waxyeelada Entotoxin) iyo Toxin B (Cytotoxin). Qaar ka mid ah noocyada ayaa soo saara toxins labadaba a iyo b, qaar kale waxay soo saaraan toxin b kaliya. Doorka suuragalka ah ee sunta toxin ee xarkaha xad dhaafka ah ee cudurka loo yaqaan 'patter to putogenicicity' wali waa laga dooday. Isticmaalka dehydrogense (GDH) oo ah sumadeeye Antigen oo ah sumadeeye fayras ah ee ku-meel-gaadhka ah ee c.diftient pronages c.diftient pronates-ka c.diftient

Sidaa daraadeed, sheygan waxaa ku jira ogaanshaha isku-dhafka ku-dhajinta

TijaaboHab wax loo qabto

Keen tijaabooyin, muunad, iyo / ama kontorool heerkulka qolka (15 - 30 ° C) kahor isticmaalka.

1. Imtixaanka ka qaad jeebkeeda shaabadaysan, oo ku rid dusha nadiifka ah, heer sare. Ku calaamadee aaladda bukaanka ama kontaroolka. Natiijooyinka ugu wanaagsan ee ay tahay in lagu sameeyo hal saac gudahood.
2. Diyaarinta muujinta:

Ka fur daboolka tuubada tubada aruurinta ee muunada ah, ka dibna si aan kala sooc lahayn u xasilin usha saamiga muunadda saxarada ugu yaraan 3 goobood oo kala duwan si aad u aruuriso qiyaasta saxarada (u dhiganta 1/4 ee digir). Ku hay tuubooyinka qarashka aruurinta ee leh soo saarista khaanadaha si toos ah, geli usha sambabka, tuuji hoosta tuubada. Ku qas saxarada saxarada si fiican u ruxso adiga oo ruxaya dhalada dhawr ilbidhiqsiyo ah.

3. Habka Assay:

• Ku adkee daboolka tuubada aruurinta tubada ee muunada, ka dibna rux tubada tubada aruurinta si adag si aad ugu qasto muunada iyo xayiraada soosaarka. Tuubada ka tag tubada keligaa 2 daqiiqo.
• Ka saar daboolka yar dusha sare.
• Dhalada ku hay meel toosan oo ku saabsan muunadda ceelka kaadhka tijaabada, gaarsiinta 3 dhibcood (qiyaastii 90μl) ee saxarada saxarada ee muunad (yaasha) oo aad bilowdo saacadaha.

Xusuusin: Ka fogow xarkaha hawo-mareenka hawada ee muunada (yaasha), oo ha ku darin wax xal ah oo ku aadan natiijada natiijada.

Maaddaama tijaabadu ay bilaabaneyso inay shaqeyso, midabku wuxuu u guuri doonaa natiijada ka dhasha aagga bartamaha aaladda.

• Sug sugitaanka faashadda midabka leh si ay u muuqdaan. Akhri natiijada u dhaxaysa 5 - 10 daqiiqo. Muunad adag oo toosan ayaa laga yaabaa inay muujiso natiijada hore. Ha u fasiro natiijada ka dib 15-kii imtixaanku bilaabmayo inuu shaqeeyo, midabku wuxuu u haajiraa doonaa natiijooyinka natiijada ee xarunta aaladda

Tarjumida Natiijooyinka

Qalliinka 'Clouttridium' gdhh

• Saxeex (+):Laba casaan oo guduudan ayaa muuqan. Mid wuxuu ku yaal aagga ogaanshaha (t) kan kale wuxuu ku yaal aagga xakamaynta tayada (c).

Xusuusin:Kooxda gaduudka guduudan ee aagga ogaanshaha (t) waxay muujin kartaa ifafaale midab madow iyo khafiif ah. Si kastaba ha noqotee, inta lagu gudajiro waqtiga kormeerka ee cayiman, iyadoon loo eegayn midabka kooxda, xitaa koox aad u jilicsan waa in loo fasiraa natiijo wanaagsan.

• Diidmo (-):Kaliya casaan cagaaran oo guduudan ayaa ka muuqata aagga xakameynta tayada (c). Looma helin xargaha casaan guduudan ee guduudan laga helay aagga ogaanshaha (t). Natiijooyin diidmo ah ayaa muujinaya in aan wax laga diro
• Cillad la'aan:Ma jiraan koox casaan casaan ah oo ku yaal aagga kontaroolka tayada leh (c). Waxay muujineysaa qalliin qaldan ama ka sii daraya imtixaanka. Xaaladdan oo kale, u aqri tilmaamaha si taxaddar leh oo dib ugu celi tijaabo cusub. Haddii dhibaatadu sii socoto, waa inaad isla markiiba joojisaa isticmaalka lambarka dufcadda oo aad la xiriirto alaab-qeybiyahaaga deegaankaaga.

CLIMTRIDITIONITINS Toxin a iyo toxin b

• Toxin aTogan: Xariiqda midabka leh ee xarunta safka kontaroolada (c) ayaa muuqata, oo xariiq midab leh ayaa ka muuqata khadka khadka imtixaanka T1. Natiijadu waxay tahay mid togan oo ku habboon iskuxirka ku-xigta
• ToxinB Togan:Xariiqda midabka leh ee xarunta safka kontaroolada (c) ayaa muuqata, oo xariiq midab leh ayaa ka muuqata khadka khadka imtixaanka T2. Natiijadu waxay tahay mid togan oo ku habboon ku-dhowaad Clombridium Corporta Toxin Band Tooska ah ayaa muujinaya infekshanka iskuxirka.
• Toxin a&B Togan:Xariiqda midabka leh ee xarunta safka kontaroolada (c) ayaa muuqata, iyo laba sadar midab oo midab leh waa inay ka soo baxaan gobollada tijaabada ee T1and T2. Awoodda midabka ee khadadka ma aha inay isbarbar dhigaan. Natiijadu waxay tahay mid togan oo ku saabsan sunta toxin a & toxin b oo waxay muujineysaa kala-sooca iskuxirka
• Diidmo (-):Kaliya casaan cagaaran oo guduudan ayaa ka muuqata aagga xakameynta tayada (c). Lama helin xargaha casaan guduudan ee guduudan laga helay aagga ogaanshaha (T1) iyo (T2). Natiijooyin diidmo ah ayaa muujinaya in aan wax laga diro
• Cillad la'aan:Ma jiraan koox casaan casaan ah oo ku yaal aagga kontaroolka tayada leh (c). Waxay muujineysaa qalliin qaldan ama ka sii daraya imtixaanka. Xaaladdan oo kale, u aqri tilmaamaha si taxaddar leh oo dib ugu celi tijaabo cusub. Haddii dhibaatadu sii socoto, waa inaad isla markiiba joojisaa isticmaalka lambarka dufcadda oo aad la xiriirto alaab-qeybiyahaaga deegaankaaga.

• Astaamaha waxqabadka

  Dareenka caafimaad, gaar ahaaneed iyo sax ahaanta

  Ku-dhowaad xildhibaanka GDH, Toxin A iyo Toxin B Com Combo Combod Tijaabada degdega ah ee loo adeegsaday PCR sida habka tixraaca. Natiijooyinka waxay muujiyeen in ku-dhowaa 'Clitiontridium-ka' Wemptridium GDH, Toxin a iyo Toxin B Tijaabada Degdega ah ee Combo ee Combo ay leeyihiin dareen sare iyo cayiman.

  Jadwalka:CLIMTRIDITIONITINS GDH AntigenenDhakhso ahTijaabo vs.Rt - pcr  

  Hab		Rt - pcr		Wadarta natiijooyinka
  CLIMTRIDITIONITINS GDH Antigenen Dhakhso ahTijaabo	Natiijala	Xaqiiq ah	Diidmo ah
  	Xaqiiq ah	120	3	123
  	Diidmo ah	6	312	318
  Natiijada wadarta		126	315	441

  Xasaasiyadda qaraabada: 95.24% (95% CI: 89.92% ~ 98.23%)

  Xaddisoolinimada qaraabada: 99.05% (95% CI: 97.24% ~ 99.80%)

  Saxsanaanta: 97.96% (95% CI: 96.16% ~ 99.06%)

  Jadwalka: Sumpridium sunta tijaabada degdega ah ee antigen vs.Rt - pcr  

  Hab		Rt - pcr		Wadarta natiijooyinka
  Sumpridium sunta tijaabada degdega ah ee antigen	Natiijala	Xaqiiq ah	Diidmo ah
  	Xaqiiq ah	111	5	116
  	Diidmo ah	9	109	118
  Natiijada wadarta		120	114	234

  Xasaasiyadda qaraabada: 92.50% (95% CI: 86.24% ~ 96.51%)

  Qeexida qaraabada: 95.61% (95% CI: 90.06% ~ 98.56%)

  Saxsanaanta: 94.02% (95% CI: 90.17% ~ 96.69%)

  Jadwalka: The Contridium Toxhin B Imtixaanka Degdegga ah vs.Rt - pcr  

  Hab		Rt - pcr		Wadarta natiijooyinka
  Clouttridium Toxin B Imtixaanka Degdegga ah	Natiijala	Xaqiiq ah	Diidmo ah
  	Xaqiiq ah	99	4	103
  	Diidmo ah	4	114	118
  Natiijada wadarta		103	118	221

  Xasaasiyadda qaraabada: 96.12% (95% CI: 90.35% ~ 98.93%)

  Qeexida qaraabada: 96.61% (95% CI: 91.55% ~ 99.07%)

  Saxsanaanta: 96.38% (95% CI: 87.43% ~ 95.10%)

  Iskutallaab - falcelinta

  Qiimeyn ayaa la sameeyay si loo go'aamiyo iskutallaabta khilaafaadka iskutallaabta ku-meel-gaarka ah ee isku-dhafka ku-xigta Majiro falcelin iskutallaab ah oo ka dhan ah cudurada caloosha ee caloosha marmarka qaarkood sida soo socda:

   

  Campylobact coli	Shigella dysoneriae	Salmonella Entertidid
  Campylobact Jeejuni	Shigella flexnei	Salmonelra pattyphi
  E.Coli O157: H7	Shigella Sonei	Salmonella Typhi
  H.pylori	Salmonella Typhimurium	Staphylococcus aureus
  Listera MonocyTogenes	Shigella Boydi	Yoersinia locotoliticata

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