Synthetic Marijuana (K2) Rapid Test (Urine)

Synthetic cannabis is a psychoactive herbal and chemical product that,when consumed, mimics the effects of cannabis. It is best known by thebrand name K2 and Spice, both of which have largely become genericized trademarks used for refer to any systhetic cannabis product. The studiessuggest that synthetic cannabinoid intoxication is associated with acute psychosis, worsening of previously stable psychotic disorders, and also may have the ability to trigger a chronic (long-term) psychotic disorder among vulnerable individuals such as those with a family history of mental illness. A large and complex variety of synthetic cannabinoids, most ofter cannabicyclohexanol, JWH-018, JWH-073, or HU-210, are used. As of March 1, 2011, five cannabinoids, JWH-018, JWH-073, CP-47, JWH-200 and cannabicyclohexanol are illegal in US because these substances have the potential to be extremely harmful and, therefore, pose an imminent hazard to the public safety.

MATERIALS

Materials Provided

Materials Required but Not provided

PROCEDURE

Bring tests, specimens, and/or controls to room temperature (15-30°C) before use.

1. 1. Remove the test from its sealed pouch, and place it on a clean, level surface. Label the test with patient or control identification. For best results, the assay should be performed within one hour.
2. 2. Using the provided disposable pipette, transfer 3 drops of specimen (approximately 120 µL) to the specimen well (S) of the device and start the timer.

Avoid trapping air bubbles in the specimen well (S), and do not add any solution to the result area.

3. As the test begins to work, color will migrate across the membrane.

3. Wait for the colored band(s) to appear. The result should be read at 5 minutes. Do not interpret the result after 8 minutes.

4. INTERPRETATION OF RESULTS

   POSITIVE: Only one colored band appears, in the control region (C). No colored band appears in the test region (T). A positive result indicates that the drug concentration exceeds the detectable level .

    

   NEGATIVE: Two colored bands appear on the membrane. One band appears in the control region (C) and another band appears in the test region (T). A negative result indicates that the drug concentration is below the detectable level .

    

   INVALID: Control band fails to appear. Results from any test which has not produced a control band at the specified read time must be discarded. Please review the procedure and repeat with a new test. If the problem persists, discontinue using the kit immediately and contact your local distributor.

   NOTE:

   1. The intensity of color in the test region (T) may vary depending on the concentration of analytes present in the specimen. Therefore, any shade of color in the test region (T) should be considered negative. Please note that this is a qualitative test only, and cannot determine the concentration of analytes in the specimen.
   2. Insufficient specimen volume, incorrect operating procedure or expired tests are the most likely reasons for control band failure.

   3. LIMITATIONS OF THE TEST

      1. 1. The Synthetic Marijuana (K2) Rapid Test(Urine) is for professional in vitro diagnostic use, and should be only used for the qualitative detection of
      2. 2. This assay provides a preliminary analytical test result only. A more specific alternative chemical method must be used in order to obtain a confirmed analytical result. Gas chromatography/mass spectrometry (GC/MS) has been established as the preferred confirmatory method by the National Institute on Drug Abuse (NIDA). Clinical consideration and professional judgment should be applied to any test result, particularly when preliminary positive results are indicated.
      3. 3. There is a possibility that technical or procedural errors as well as other substances and factors may interfere with the test and cause false results.
      4. 4. Adulterants, such as bleach and/or alum, in urine specimens may produce erroneous results regardless of the analytical method used. Therefore, please preclude the possibility of urine adulteration prior to testing.
      5. 5. A positive result indicates the presence of a K2 only, and does not indicate or measure intoxication.
      6. 6. A negative result does not at any time rule out the presence of K2 in urine, as they may be present below the minimum detection level of the test.
      7. 7. The test results can be used to provide evidence and basis for therapy and treatment plans of drug dependence and toxic psychosis. For laboratory professional in vitro diagnostic use only.
      8. 8. The test should only be performed by health professionals in a clinical/hospital setting to aid in screening of drug of abuse to determine the follow-up treatment measures in combination of clinical symptoms.
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