ICampylobacter yovavanyo lwe-Antign

Ukusetyenziswa kwenjongo

ICampylobacter Vigen Uvavanyo lwe-Antigen I-Anticn Viest kuvavanyo olukhawulezileyo lwe-chromassic ye-chromassic yokuchongwa komgangatho wekampanga ye-SPP. Antign kwisitulo somntu. Iziphumo zovavanyo zenzelwe ukunceda ekuqaleni - ukufumanisa isifo sosulelo lweCampylobacter kwizigulana ezineempawu zokukrokrelwa kwe-gestroenteritis ezibangelwa yiCampslobaerter.

IsixhoboS

Izixhobo ezibonelelweyo

Izinto ezifunekayo kodwa azibonelelwanga

VavanyoInkqubo

Yiza novavanyo, iisampulu, kunye / okanye ulawulo kubushushu begumbi (15 - 30 ° C) ngaphambi kokusebenzisa.

1. Susa uvavanyo kwingxowa yayo etywiniweyo, kwaye uyibeka kumphezulu ococekileyo, wenqanaba. Faka ilebhile ifowuni kunye nokuchongwa kwesigulana okanye ukulawula. Ngenxa yeziphumo ezilungileyo i-ASAST kufuneka yenziwe ngaphakathi kwiyure enye.
2. Ukulungiswa okutsha

Ungayiniki ibhotile yesampula, sebenzisa intonga ye-App iqhotyoshelweyo eqhotyoshelweyo kwi-cap yokudlulisela isiqwenga esincinci (4 - 6 mm kwi-Buffer Tube equkethe i-Buffer ye-Asffle. Ngolwelo okanye uSemi - Izitulo eziqinileyo, zongeza i-100 i-chinaroliter yesitulo kwi-vial ngombhobho ofanelekileyo. Faka intonga kwityhubhu kwaye uqinise ngakumbi. Umxube isampulu ye-stool kunye ne-buffer ngokucacileyo ngokucheba ibhotile kwimizuzwana embalwa.

3. Inkqubo ye-Assay

3.1 Bamba i-Assay Buffer Tub the nkqo ngenqaku eliya kwicala kude novavanyo, phuma kwincam.

3.2. Bamba ibhotile kwisikhundla esithe nkqo ngaphezulu kwesampulu yekhadi lovavanyo, uhambise amaconsi ama-3 (120 - i-70 μl) yesampulu yesitulo esihlanjelweyo kwimodeli (s) kwaye uqalise ixesha.

Kulumkele ukubopha iibhubhu ze-air kwi-specian kakuhle (s), kwaye akongezi nasiphi na isisombululo kwisiphumo.

Njengoko uvavanyo luqala ukusebenza, umbala uza kuqhutywa kwindawo yonke kwiziphumo embindini wesixhobo.

3.3. Lindela iqela lemibala (s) lokuvela. Funda isiphumo phakathi kwe-5 - Imizuzu eli-10. Isampulu eyomeleleyo enamandla inokubonisa kwangaphambili.

Musa ukutolika iziphumo emva kwemizuzu eli-10.

Njengoko uvavanyo luqala ukusebenza, umbala uza kuqhutywa kwindawo yonke kwiziphumo embindini wesixhobo.

Ukutolikwa kweziphumo

I-Hough: Mbini Iibhendi ezinemibala zivela kwi-membrane. Ibhendi enye ivela kwingingqi yolawulo (c) kwaye enye ibhendi ivela kummandla wovavanyo (t).

I-negating: Ibhendi enye kuphela ivela kwingingqi yolawulo (c).Akukho bandi zibonakalayo zibonakalayo kwingingqi yovavanyo (t).

Ayisebenzi: Ibhendi yolawulo iyasilela ukuvela.Iziphumo ezivela kulo naluphi na uvavanyo olungavelisi ibhendi yolawulo ngexesha lokufunda elichaziweyo kufuneka lilahlwe. Nceda ujonge inkqubo kwaye uphinde uphinde ngovavanyo olutsha. Ukuba ingxaki iyaqhubeka, ukuyeka ukusebenzisa i-kit kwangoko kwaye uqhagamshelane nomsasazi wakho wengingqi.

Iimpawu zentsebenzo

Itheyibhile: ICampylobacter Antigen Vide Vid Vs. Inkcubeko

Imvakalelo yesihlobo: 98.51% (95% CI: 91.25% ~> 99.99%)

Ukuchaneka kwe-97.89% (95% CI: 95.62% ~ 99.06%)

Ukuchaneka: I-97.99% (95% CI: 96.02% ~ 99.05%)