Clostridium difficile Antigen Rapid Test

I-Clotoridium diatidile antign yovavanyo olukhawulezayo

Inkcazo emfutshane:

Isetyenziselwe: ngokufunyanwa okulinganayo kwe-Glutamate Dehydrogeses

I-Specimen: Isitulo somntu

Isiqinisekiso:CE

I-Moq:1000

Ixesha lokuzisa:I-2 - Iintsuku ezi-5 emva kwentlawulo

Ukupakisha:Iimvavanyo ezingama-20 zeekhithi / ibhokisi yokupakisha

Beka ubomi kwishelufa:Iinyanga ezingama-24

Intlawulo:I-T / T, I-Western Union, PayPal

Ixesha le-Assay: 10 - Imizuzu eli-15


Iinkcukacha zemveliso

Iimpawu zeMveliso

Ukusetyenziswa kwenjongo

I-Clotoridium diatirile. Iziphumo zovavanyo zenzelwe ukunceda ekufumaneni i-antibiotic - ehambelana ne-cruarhea colitis kunye ne-pseudomembranous colitis ebangelwa lusulelo kunyeI-Clotoridium diatirile.

Umgaqo

Usebenzisa umgaqo-siseko we-Colloidal Gollochiromatographromatographrographrography, ibhokhwe i-anti - i-polyclonl antidclon antids antidy (uthotho c) kunye ne-anti -I-Clotoridium diatirileI-monoclonional antidy idityaniswe kwifitutha ifilimu. Imouse ye-monoclonn antibodies ukuyaC. disticilebalungiswa kwiipleyiti zegolide ezineelebheli zegolide. Xa isampulu eyakhayo iyavavanywa,I-Clotoridium diatirileI-Antign kwiSampulu ibophelela kwimouseI-Clotoridium diatirileI-monoclonn antidy the ping yegolide, esenza ubunzima obuhamba ecaleni kwe-membrane nge-chromatography. Emva komgca wokuqaphela, yenza i-sandwich ye-sandwich kunye ne-antids otsids yophuhliso lwemibala, kwaye idityaniswa ne-Anti - Iisampulu zemouse zibonisa kuphela kumgca wokunqongophala komgangatho.

Inkqubo yovavanyo

Yiza novavanyo, iisampuli, kunye / okanye ulawulo kubushushu begumbi (15 - 30 ° C)ngaphambi kokusetyenziswa.

  1. Susa uvavanyo kwingxowa yayo etywiniweyo, kwaye uyibeka kumphezulu ococekileyo, wenqanaba. Faka ilebhile ifowuni kunye nokuchongwa kwesigulana okanye ukulawula. Ngenxa yeziphumo ezilungileyo i-ASAST kufuneka yenziwe ngaphakathi kwiyure enye.
  1. Ukulungiselela:

Ungayikhupheli ityhubhu ye-cube yengqokelela, emva koko ixhaphake ngokungagungqiyo kwi-Sampling ye-Sampling kwisitulo esinezitimemitha ezi-3 ezahlukeneyo zokuqokelela izitolo (ezilinganayo kwi-1/4 ye-PEA). Bamba iityhubhu zokuqokelelwa kwecawa nge-buffer the therch ngokuthe nkqo, faka i-Sampling Rod, tyibilika ezantsi kwetyhubhu. Hlanganisa i-Stool Stool kunye ne-buffer ngokufanelekileyo ngokucheba ibhotile kwimizuzwana embalwa.

  1. Inkqubo ye-Assay:

 Qinisa i-cap kwi-tube yengqokelela ye-cube, emva koko nyikima i-tube yengqokelela yamandla ukuxubana ne-buffer kunye ne-buffer yokukhupha. Shiya ityhubhu yedwa kwimizuzu emi-2.

Susa isiciko esincinci phezulu.

 Bamba ibhotile kwindawo ethe nkqo ngaphezulu kwesampulu yequmrhu lovavanyo, ukuhambisa amathontsi ama-3 (malunga ne-90l) yesampulu yesitulo esiqengqelekayo kwimodeli (s) kwaye uqalise ixesha.

Phawula:Kulumkele ukubopha iibhubhu ze-tubs ze-air kwi-Specian kakuhle (s), kwaye akongezi nasiphi na isisombululo kwisiphumo sesiphumo.

Njengoko uvavanyo luqala ukusebenza, umbala uza kuqhutywa kwingingqi yesiphumo embindini wesixhobo.

 Lindela iqela lemibala (s) lokuvela. Funda isiphumo phakathi kwe-5 - Imizuzu eli-10. Isampulu eyomeleleyo enamandla inokubonisa kwangaphambili. Musa ukutolika iziphumo emva kwemizuzu eli-15. Njengoko uvavanyo luqala ukusebenza, umbala uya

Ukufuduka ngaphaya kwendawo yesiphumo embindini wesixhobo.

Ukutolikwa kweziphumo

IQHELEKILE (+):Iibhendi ezibomvu ezibomvu zivela. Inye ikwindawo yokuchonga (T), enye ikwingingqi yolawulo oluphambili (c).

Phawula:Ibhendi ebomvu emfusa kwingingqi yokuchonga (t) inokubonisa i-phenomenon yebala elimnyama nelikhanyayo. Nangona kunjalo, ngexesha lokujonga okuchaziweyo, ngaphandle kombala weqela, kunye neqela elibuthathaka kakhulu

kufuneka itolikwe njengesiphumo esifanelekileyo.

Gwenxa (-):Ibhendi ebomvu kuphela ivela kwingingqi yoLawulo loMgangatho (c). Akukho bands ezibomvu ezifunwayo kwingingqi yokuchonga (t). Isiphumo esibi sibonisa hayiI-Clotoridium diatirileusulelo.

Ayisebenzi:Akukho band ibomvu i-purple kwingingqi yolawulo olukumgangatho (c). Ibonisa ukusebenza okungachanekanga okanye ukonakala kovavanyo. Kule meko, funda imiyalelo ngononophelo kwakhona kwaye uphinde ucinge ngovavanyo olutsha. Ukuba ingxaki

Ukuqhubeka, kuya kufuneka uyeke kwangoko usebenzisa inombolo ye-batch kwaye uqhagamshelane nomthengisi wakho wasekhaya

Umda

  1. 1. II-Clotoridium diatirile(GDH) Uvavanyo olukhawulezileyo lwe-Antign luyingcaliin vitroUkusetyenziswa koxilongo, kwaye kufuneka isetyenziselwe ukufunyanwa komlinganiso womntuI-Clotoridium diatirile.
  1. 2. Iziphumo zovavanyo kufuneka zisetyenziswe kuphela ukuvavanya isigulana ngemiqondiso kunye neempawu zesifo. Isifo esiqinisekileyo seklinikhi kufuneka senziwe kuphela ngugqirha emva kokuba yonke iKlinikhi yeKlinikhi iphononongwe.
  1. I-3.

4. Iisampuli ezivela kwizigulana ezifumene amalungiselelo ee-antibodies ze-monoclon Ezo zinto zinokubangela iziphumo ezingezizo ezingezizo okanye ezibubuxoki.

  1. I-5. Njengoko lonke uvavanyo lokuqonda isifo, isifo esiqinisekisiweyo kufuneka senziwe kuphela ngugqirha emva kokuba kufundwe zonke iicawa kunye neziphumo zelebhu.

 



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