I-cocaine (coc) yovavanyo olukhawulezayo (umchamo)

Ithathwe kumagqabi e-coca isityalo, i-cacaine yinkqubo ephakathi ye-nervouse inyulu kunye ne-anesthetic yendawo. Iziphumo zomzimba nezengqondo zokusetyenziswa kwe-cocaine zibandakanya izinga lentliziyo, umkhuhlane, ukudinwa komfundi, i-diaphoreris, i-diaphoreris, euphoria kunye nokunyuka kwamandla. Ngokwenyani, icocaine icinyiwe ngokukhawuleza kwi-Benzoycgonine. Isiqingatha - Ubomi be-Benzoycgonine (5 ukuya kwi-8 iiyure) bude kakhulu kune-Cacaine yomzali (i-0.5 ukuya kwi-1.5). I-Benzaycgonine inokufunyanwa kumchamo ukuya kwiintsuku ezi-3 emva kokusetyenziswa kwecocaine.

VavanyoInkqubo

Yiza novavanyo, iisampunzi, i-buffer kunye / okanye ulawulo kubushushu begumbi (i-15 30 ° C) ngaphambi kokusebenzisa.

1. 1. Susa uvavanyo kwingxoxo yayo etywiniweyo, okanye ususe umtya omnye kwi-canister, kwaye uyisebenzise ngokukhawuleza. Ngenxa yeziphumo ezilungileyo, i-assay kufuneka yenziwe ngaphakathi kwiyure enye. I-USETS kufuneka ivaliwe ngokuqinileyo emva kokususa imitya.
2. 2. Bamba umtya ngokuphela, apho igama lemveliso lishicilelwe. Ukuthintela ungcoliseko, musa ukuchukumisa i-membrane.
3. 3. Ukubamba umtya ngokuthe nkqo, dip i-gip yovavanyo kwi-Urneimen ye-10 - Imizuzwana eli-15. Sukungena edlula ilayini ephezulu (i-max) kumtya wovavanyo.
4. 4. Emva kokuba uvavanyo lugqibile ukuqhuba, susa umtya ovela kwi-strackimen kwaye uyibeke kwindawo engeyiyo - i-absorblerbler. Qala ixesha kwaye ulinde iqela lemibala (s) livele. Isiphumo kufuneka sifundwe kwimizuzu emi-5. 5. Sukutolika iziphumo emva kwemizuzu engama-8.

5. Ukutolikwa kweziphumo
   

   I-Hough: Inye kuphela ibhendi enemibala ebonakalayo, kwingingqi yolawulo (c).Akukho bandi zibonakalayo zibonakalayo kwingingqi yovavanyo (t).

    

   I-negating:Iibhendi ezimbini ezinemibala zivela kwi-membrane. Ibhendi enye ivela kwingingqi yolawulo (c) kwaye enye ibhendi ivela kummandla wovavanyo (t).

    

   Ayisebenzi: Ibhendi yolawulo iyasilela ukuvela.Iziphumo ezivela kulo naluphi na uvavanyo olungavelisi ibhendi yolawulo ngexesha lokufunda elichaziweyo kufuneka lilahlwe. Nceda ujonge inkqubo kwaye uphinde uphinde ngovavanyo olutsha. Ukuba ingxaki iyaqhubeka, ukuyeka ukusebenzisa i-kit kwangoko kwaye uqhagamshelane nomsasazi wakho wengingqi.

   PHAWULA:

   1. Ubunzulu bombala kuMmandla wovavanyo (T) bungahluka ngokuxhomekeka kuxinzelelo lwehlalutyishini ekhoyo kwinqaku. Ke ngoko, nawuphi na umthunzi wombala kummandla wovavanyo kufuneka uthathwe njengento entle. Qaphela ukuba olu luvavanyo olufanelekileyo kuphela, kwaye awukwazi ukufumanisa ukugxininiswa kohlalutyiweyo ku-Speciamen.
   2. Ivolumu engonelanga, inkqubo yokusebenza engachanekanga okanye iimvavanyo eziphelelwe lixesha zezona zizathu zibalulekileyo zokungaphumeleli kwebhendi.

   3. Izixhobo

      Izixhobo ezibonelelweyo

      · I-chict nganye yovavanyo

      ·  Iphakheji

      Izinto ezifunekayo kodwa azibonelelwanga

      · Ulawulo oluqinisekileyo nolungalunganga

      · Ixesha

      · I-Centrifuge

   Imida
   

   1. 1. I-cocaine (coc) yovavanyo olukhawulezileyo (umchamo) yingcalii-In vitro Ukusetyenziswa kwamanani, kwaye kufuneka isetyenziselwe kuphela ukutshintshwa kwecocaine.
   2. 2.Le Assay ibonelela ngesiphumo sovavanyo lovavanyo lwangaphambili kuphela. Enye indlela ekhethekileyo yekhemikhali kufuneka isetyenziswe ukuze ufumane iziphumo eziqinisekisiweyo. I-chromatography / i-spectrometry (GC / MS) isekwa njengendlela ekhethiweyo yokuqinisekisa liziko likazwelonke le-National Attion (iNida). Ukuqwalaselwa kweklinikhi kunye nomgwebo wobungcali kufuneka kusetyenziswe nakwesiphi na isiphumo sovavanyo, ngakumbi xa iziphumo ezilungileyo zokuqala zibonisiwe.
   3. 3.Kaloku kunokwenzeka ukuba iimpazamo zezobuchwephesha okanye zenkqubo kunye nezinye izinto zinokuphazamisa uvavanyo kwaye zibangela iziphumo zobuxoki.
   4. Ii-Bleasterants, ezinjenge-bleach kunye / okanye i-alum, kwimizobo yomchamo inokuvelisa iziphumo eziphosakeleyo nokuba yeyiphi na indlela yohlalutyo esetyenzisiweyo. Ke ngoko, nceda uthintele ukubakho kokuThembisa umchamo ngaphambi kovavanyo.
   1. 5. Isiphumo esivakalayo sibonisa ubukho be-cocaine kuphela, kwaye ayibonisi okanye ilinganisele ukunxila.
   2. 6. Isiphumo esibi asinalo nangaliphi na ixesha ubukho be-cocaine kumchamo, njengoko benokubakho ngezantsi kwenqanaba lokufumanisa kovavanyo.
   3. 7.Imva kovavanyo alwenzi phakathi kwecocaine kunye namayeza athile.
   4. 8. Iziphumo zovavanyo zinokusetyenziselwa ukubonelela ubungqina nesiseko sonyango kunye nezicwangciso zonyango zokuxhomekeka kweziyobisi kunye nengqondo enetyhefu. I-laboratory yengcali kwi-vitro yokusetyenziswa kuphela.
   5. 9. Uvavanyo kufuneka lwenziwe kuphela ngabaqeqeshi bezempilo kwiklinikhi / sendawo yesibhedlele ukunceda ekuhlolweni lweziyobisi ukumisela la manyathelo - Ukunyuka kwamanyathelo onyango ngokudibanisa iimpawu zeklinikhi.
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