I-Cryptosposporidium Antigen yovavanyo olukhawulezayo

Ukusetyenziswa kwenjongo

I-Cryptosposporiririum parvm Antign i-Antign i-chromasgraphic i-chromassic i-chidmoospographic yokuchongwa komgangatho we-cryptospopridium ye-rigtosporirium. Iziphumo zovavanyo zenzelwe ukunceda ekufumanekeni kwesifo sosulelo lwe-cryptosporidium losulelo kunye nokubeka iliso ekusebenzeni konyango.

Intshayelelo

I-cryptosposiphesposiosis sisifo esosulelayo esibangelwa yi-cryptosporidium parvum, eyenziwa ngamaxesha ngamaxesha ezinye iintlobo ze-cryptosporisium. Iimpawu zihlala zivela malunga neentsuku ezisi-7 emva kosuleleko, kubandakanya nentlungu yesisu, urhudo olwenziweyo, ukugabha kunye nefiva. Iimpawu zezigulana ezininzi zihlala iintsuku ezi-6 ukuya kwezi-10, kodwa zisenokuhlala iiveki ezininzi. Izigulana ezinengxaki yokuzikhusela, ezinjengokusuleleka, zinokuba zinzulu kakhulu kwaye nobomi - isoyikisa. Ukususela kwingxelo esemthethweni ngo-1976, ifumanise ukuba isifo sixhaphake, kwaye yi-pathogen eqhelekileyo yesakhelo yabakhenkethi. Zininzi izigulana ezinesifo sikagawulayo nesi sifo.

Inkqubo yovavanyo

Yiza novavanyo, iisampulu, kunye / okanye ulawulo kubushushu begumbi (15 - 30 ° C) ngaphambi kokusebenzisa.

1. Susa uvavanyo kwingxowa yayo etywiniweyo, kwaye uyibeka kumphezulu ococekileyo, wenqanaba. Faka ilebhile ifowuni kunye nokuchongwa kwesigulana okanye ukulawula. Ngenxa yeziphumo ezilungileyo i-ASAST kufuneka yenziwe ngaphakathi kwiyure enye.
2. Ukulungiswa okutsha

Unscrew the sample bottle, use the attached applicator stick attached on the cap to transfer small piece of stool (4-6 mm in diameter; approximately 50 mg – 200 mg) into the sample bottle containing specimen preparation buffer . Ngolwelo okanye uSemi - Izitulo eziqinileyo, zongeza i-100 i-chinaroliter yesitulo kwi-vial ngombhobho ofanelekileyo. Faka intonga kwibhotile kwaye uqinise ngakumbi. Umxube isampulu ye-stool kunye ne-buffer ngokucacileyo ngokucheba ibhotile kwimizuzwana embalwa.

3. Inkqubo ye-Assay

3.1 Bamba isampuli yebhotile ngokuthe tye ngenqaku lencam kwicala lokuya kwicala lokuvavanywa, phuma kwincam.

3.2. Bamba ibhotile kwisikhundla esithe nkqo ngaphezulu kwesampulu yekhadi lovavanyo, uhambise amaconsi ama-3 (120 - i-70 μl) yesampulu yesitulo esihlanjelweyo kwimodeli (s) kwaye uqalise ixesha.

Kulumkele ukubopha iibhubhu ze-air kwi-specian kakuhle (s), kwaye akongezi nasiphi na isisombululo kwisiphumo.

Njengoko uvavanyo luqala ukusebenza, umbala uza kuqhutywa kwindawo yonke kwiziphumo embindini wesixhobo.

3.3. Lindela iqela lemibala (s) lokuvela. Funda isiphumo phakathi kwe-5 - Imizuzu eli-10. Isampulu eyomeleleyo enamandla inokubonisa kwangaphambili.

Musa ukutolika iziphumo emva kwemizuzu eli-10.

Njengoko uvavanyo luqala ukusebenza, umbala uza kuqhutywa kwindawo yonke kwiziphumo embindini wesixhobo.

Ukutolikwa kweziphumo

I-Hough: Mbini Iibhendi ezinemibala zivela kwi-membrane. Ibhendi enye ivela kwingingqi yolawulo (c) kwaye enye ibhendi ivela kummandla wovavanyo (T).

I-negating: Ibhendi enye kuphela ivela kwingingqi yolawulo (c).Akukho bandi zibonakalayo zibonakalayo kwingingqi yovavanyo (t).

Ayisebenzi: Ibhendi yolawulo iyasilela ukuvela.Iziphumo ezivela kulo naluphi na uvavanyo olungavelisi ibhendi yolawulo ngexesha lokufunda elichaziweyo kufuneka lilahlwe. Nceda ujonge inkqubo kwaye uphinde uphinde ngovavanyo olutsha. Ukuba ingxaki iyaqhubeka, ukuyeka ukusebenzisa i-kit kwangoko kwaye uqhagamshelane nomsasazi wakho wengingqi.

1. ULAWULO LWEMEKO

   • Ulawulo lwenkqubo lwangaphakathi lubandakanyiwe kuvavanyo. Ibhendi enemibala evelayo kwingingqi yolawulo (c) ithathwa njengolawulo lwenkqubo yangaphakathi, eqinisekisa ivolumu eyoneleyo kunye nenkqubo yenkqubo efanelekileyo.
   • Ulawulo lwangaphandle alubonelelwanga ngale khit. Kucetyiswa ukuba ulawulo olululo nolungalunganga luvavanywe njengeziqhelaniso zelebhu ezilungileyo ukuqinisekisa inkqubo yovavanyo kunye nokuqinisekisa ukusebenza kakuhle kovavanyo.

UKUHLAZIYWA KOKUVAVANYA

1. I-Tracryptosposhirium parvum ye-Antign I-Antign yovavanyo lwe-vitro yokusebenzisa, kwaye kufuneka isetyenziselwe ukufunyanwa komgangatho we-cryptosporisium.
2. Iziphumo zovavanyo kufuneka zisetyenziswe kuphela ekuvavanyweni kwisigulana ngemiqondiso kunye neempawu zesifo. Isifo esiqinisekileyo seklinikhi kufuneka senziwe kuphela ngugqirha emva kokuba yonke iKlinikhi yeKlinikhi iphononongwe.
3. Njengakwi-antibodies esebenzisa ii-antibodies ze-antibodies zemouse, ukubakho malunga nokuphazamiseka kwe-anti - antibodies zemouse (i-hama) kwinqaku. Iisampulu ezivela kwizigulana ezifumene amalungiselelo ee-antibodies ze-monoclon Ezo zinto zinokubangela iziphumo ezingezizo ezingezizo okanye ezibubuxoki.

• Njengoko lonke uvavanyo lokuqonda isifo, isifo esiqinisekisiweyo kufuneka senziwe kuphela ugqirha emva kokuba zonke iziphumo zekliniki kunye nelebhu zivavanyiwe.