I-Dengue Igg / Igm kunye ne-NS1 combo

Inkcazo emfutshane:

Used For: for the qualitative detection of Dengue NS1 antigen and IgG/IgM antibodies to Dengue in human whole blood,serum or plasma specimen.

I-SURTEEN:Igazi liphela, i-serum okanye iplasma

Isiqinisekiso: CA

I-Moq: i-1000

Ixesha lokuhambisa: 2 - Iintsuku ezi-5 emva kwentlawulo

Ukupakisha: Iimvavanyo ezingama-20 zeKits / ibhokisi yokupakisha

Ubomi beshelup: iinyanga ezingama-24

Intlawulo: T / T, I-Western Union, PayPal

Ixesha le-Assay: 10 - Imizuzu eli-15

Nxibelelana ngoku

Iinkcukacha zemveliso

Iimpawu zeMveliso

Ukusetyenziswa kwenjongo

I-Gengue Igg / I-Igm kunye novavanyo olukhawulezayo lwe-chromassic ngokukhawuleza kukungafumani i-Dengue NS1 Antign kunye ne-Igg / i-Igm Antibodies ukuya kwigazi liphela, i-serum okanye iplasma ye-plasma.

Isishwankathelo

Intsholongwane ye-Dengoma intsholongwane, usapho lwee-irotypes ezihlukileyo ze-idas (den 1,2,3,4,4, zingatshatanga - Iphulelo le-RNA intsholongwane. Iintsholongwane zidluliselwa yi-mosquiti yexesha lasemini - Ukuluma intsapho yaseStegeTiya, ikakhulu i-Aedyes Aeypti, kunye neAedes Albopictus. Namhlanje, ngaphezulu kwe-2 lamawaka ezigidi zabantu abahlala kwiindawo ze-Asia, eAfrika, eOstreliya, kunye neMelika basengozini yosulelo lokusweleka. Iimeko eziqikelelwa ezizigidi ze-Dengue Fever kunye nama-250,000 iimeko zoBomi - Isimo se-hemorrhagic ye-hemorrhagic yenzeka minyaka le kwihlabathi liphela. Usulelo oluphambili lubangela i-antibodies ze-IGM ukwanda kwinqanaba elipheleleyo kwiintsuku ezi-3 ukuya kwezi-5 emva kokuqala komkhuhlane. I-Igm Antibodies iqhubeka kangangeentsuku ezingama-30 ukuya kwezingama-90. Uninzi lwezigulana ezithandekayo kwimimandla ye-Endemic inosulelo lwesibini, ezikhokelela kumanqanaba aphezulu e-antibodies ekhethekileyo ye-IGM ngaphambi okanye ngaxeshanye ngempendulo ye-IGM. Ke ngoko, ukufunyanwa kwe-Anti - Dengue I-Igm kunye ne-Igg Antibodies zinokukunceda ukwahlula phakathi kosulelo oluphambili nolwesibini. Mva nje, ukufunyanwa kwee-adogens ezikhutshwe ngexesha lokuphindaphinda kwentsholongwane kwisigulo esosulelekileyo kuboniswe isiphumo esithembisayo. Iyenza ukuba kufunyaniswe isifo kusuku lokuqala emva kokuqala komkhuhlane ukuya kuthi ga nge-9, kwakanye ukuba isigaba sekliniki yesi sifo sigqithile, ngenxa yoko ivumela unyango kwangoko.

I-Gengue Igg / I-Igm kunye novavanyo lwe-Com1 Comm luvavanyo olukhawulezileyo ukuba lubone ubukho bee-antibouse (i-Igm) kwi-antiens iphela, i-serum, okanye iplasma.

Izixhobo

Izixhobo ezibonelelweyo

1) Izixhobo zovavanyo

2) Ukulahla i-propper

I-3) INDAWO YOKUHLAZIYA

Izinto ezifunekayo kodwa azibonelelwanga

1) Isikhongozelo seqokelelo

2) i-lancet

3) I-Centrifuge (yePlasma kuphela)

4) Ixesha

Inkqubo yovavanyo

Vumela isixhobo sovavanyo, i-buffer, i-buffer, kunye / okanye ilawula ukulingana kubushushu begumbi (15 - 30 ° C) ngaphambi kovavanyo.

1. Yiza nengxowa kubushushu begumbi ngaphambi kokuvula. Susa isixhobo sovavanyo kwingxowa etywiniweyo kwaye uyisebenzise ngokukhawuleza.
2. Beka isixhobo sovavanyo kumgangatho ococekileyo kunye nenqanaba.

I-Dengue Igg / Igm Inxalenye

Kwi-serum okanye iisampuli zePlasma:

Bamba i-ropper ethe nkqo, zoba uphawu lwesaziso (malunga ne-5 μl), kwaye udluliselwe kwi-Storemen ye-STATE (s) yesixhobo sovavanyo, emva koko uyongeza i-droFFER (malunga ne-90 l) kwaye uqalise i ixesha. Jonga umfanekiso ongezantsi. Kulumkele ukubopha iibhubhu ze-air kwi-specian kakuhle (s).

Igazi liphela (i-veipnctionure / i-lertick) iisampulu:

Ukusebenzisa i-propper: Bamba i-Dropper ngokuthe nkqo, zoba i-Strament 0.5 - i-1 cm ngaphezulu komgca wokugcwalisa, malunga ne-10 μl) kwi-propt yegazi (i-10 μl) kwi-TAPIME COORTE, emva koko yongeza amaconsi ama-3 ye-buffer (malunga ne-90 i-UL) kwaye uqalise ixesha. Jonga umfanekiso ongezantsi.

Ukusebenzisa i-micropipette: i-pipet kunye nokuhambisa i-10 μl yegazi elipheleleyo kwi-specian ye-(s) ye-Tried, emva koko yongeza amaconsi ama-3 (malunga ne-90 μl) kwaye uqalise ixesha. Jonga umfanekiso ongezantsi.

I-Dengue NS1 Inxalenye

Ukusebenzisa i-propper: Gcwalisa iplastiki yeplastiki kunye nenqaku. Ukubamba i-Dropper ngokuthe nkqo, ukusasaza i-2 quep (malunga ne-60μl) yePlasma / i-serum i-Supep okanye i-3 ye-3

QAPHELA: Yongeza i-1 yehla kwi-saline okanye i-phosphate - i-balner buffer (i-buffers eqhelekileyo esetyenziswa kwikliniki) kwi-fessic engaqwalaselwanga kwimizuzwana engama-30 kwisiphumo sefestile, esinokwenzeka ngokubonakalayo .

3. Lindela umgca owombala ovele. Iziphumo zokufunda kwimizuzu eli-10. Musa ukutolika iziphumo emva kwemizuzu eli-15.

Ukutolikwa kweziphumo

I-Dengue Igg / Igm Inxalenye

	I-IGG iKhee: * Umgca onombala kwingingqi yelayini ye-Windows (c) ivela, kwaye ilayini enombala ivela kwingingqi ye-G. Isiphumo sinethemba lentsholongwane yeDengue) i-Gueue Sosusulelo.
	I-Igm ilungile: * Umgca onombala kwingingqi yelayini ye-Windows (c) ivela, kwaye umgca onombala uvela kwingingqi ye-Men M. Isiphumo sinethemba lentsholongwane ye-Denguee's - I-IGM AYILODS kwaye ibonisa usulelo oluphambili.
	I-IGG kunye I-Igm ilungile: * Umgca onombala kwingingqi yelayini ye-Windows (c), kwaye imigca emibini enemibala kufuneka ivele kwingingqi ye-G kunye ne-M. Isiphumo sinethemba lokuba i-IGG i-Igg Antibodies kwaye ibonisa ukosuleleka kwesibini.
	I-negating: umgca onombala kwingingqi yolawulo lwecandelo (c) iyavela. Akukho mgca ubonakala kwimimandla yovavanyo ye-1 okanye i-2 (T1 okanye i-T2).
	Ayisebenzi: Umgca wokulawula (c) usilele ukuvela. Ivolumu ye-buffer engonelanga okanye iindlela zenkqubo ezingachanekanga zezona zizathu zibalulekileyo zokusilela komgca wokusilela. Phonononga le nkqubo kwaye uphinda inkqubo ngesixhobo esitsha sovavanyo. Ukuba ingxaki iyaqhubeka, ukuyeka ukusebenzisa ikhithi yovavanyo kwangoko kwaye uqhagamshelane nomsasazi wakho wengingqi.

I-Dengue NS1 Inxalenye

IQHELEKO: Zombini ezinemibala ye-C kunye ne-T band zivela kuvavanyo kunye nengingqi yolawulo.

I-negative: inye kuphela umgca onombala kuphela ovela kwingingqi yolawulo.

Ayisebenzi: Umgca wolawulo uyasilela ukuvela. Ivolumu engonelanga okanye iindlela zenkqubo ezingachanekanga zezona zizathu zibalulekileyo zokusilela komgca. Phonononga le nkqubo kwaye uphinda uvavanyo ngesixhobo esitsha sovavanyo. Ukuba ingxaki iyaqhubeka, ukuyeka ukusebenzisa ikhithi yovavanyo kwangoko kwaye uqhagamshelane nomsasazi wakho wengingqi.