Uvavanyo lweDengue IgG/IgM ngokukhawuleza

UKUSETYENZISWA OKUHLOSIWEYO

Uvavanyo oluKhawulezayo lweDengue IgG/IgM luvavanyo olukhawulezayo lwe-chromatographic immunoassay yokukhangela umgangatho we-IgG kunye ne-IgM antibodies kwintsholongwane yeDengue egazini lomntu lonke, i-serum, okanye i-plasma njengoncedo ekuxilongweni kosulelo lweDengue olusisiseko nolwesibini.

IZIXHOBO

Izixhobo ezibonelelweyo

Izinto Eziyimfuneko Kodwa Azibonelelwanga

INKQUBO

Vumela isixhobo sovavanyo, umfuziselo, isithinteli, kunye/okanye nezilawuli ukufikelela kubushushu begumbi (15 30°C) phambi kovavanyo.

1. 1. Zisa isingxobo kwiqondo lobushushu begumbi phambi kokuvula. Susa isixhobo sovavanyo kwisingxobo esivaliweyo kwaye usisebenzise ngokukhawuleza.
2. 2. Beka isixhobo sokuvavanya kwindawo ecocekileyo kunye nenqanaba.

KubaIisampuli zeSerum okanye zePlasma: 

Bamba i-dropper ngokuthe nkqo, zoba umbonisoukuya kwiGcwalisa umgca (malunga ne-5 uL), kwaye udlulisele umboniso kumfanekiso kakuhle (S) wesixhobo sovavanyo, uze udibanise amathontsi ama-3 e-buffer (malunga ne-90 mL) kwaye uqalise isibali-xesha. Jonga umfanekiso ongezantsi. Kuphephe ukubambisa amaqamza omoya kwiqula elingumzekelo (S).

KubaIgazi Elipheleleyo (I-Venipuncture/Fingerstick) iisampulu:

Ukusebenzisa idropha: Bamba idropha ngokuthe nkqo, zoba umfuziselo0.5-1 cm ngaphezu komgca wokuZalisa, kwaye udlulisele ithontsi eli-1 legazi elipheleleyo (malunga ne-10 µL) kwisampulu yequla (S) lesixhobo sovavanyo, emva koko wongeze amathontsi ama-3 e-buffer (malunga nama-90 uL) kwaye uqalise isibali-xesha. Jonga umfanekiso ongezantsi.

Ukusebenzisa i-micropipette: I-Pipette kwaye ukhuphe i-10 µL yegazi elipheleleyo kwisampulu yequla (S) yesixhobo sovavanyo, uze wongeze amathontsi ama-3 e-buffer (malunga nama-90 µL) kwaye uqalise isibali-xesha.

3. 3. Lindela u(imi)layini onemibala ukuba ivele. Funda iziphumo ngemizuzu eyi-10. Ungatoliki iziphumo emva kwemizuzu engama-20.

4. UKUTOLIKWA KWEZIPHUMO
   
   
   

   IMIDA YOVAVANYO

   1. 1. IUvavanyo lweDengue IgG/IgM oluKhawulezayo lwenzelwei-In vitro ukusetyenziswa koxilongo kuphela. Uvavanyo kufuneka lusetyenziselwe ukufumanisa izilwa-buhlungu zeDengue kwigazi lonke, kwiserum okanye kwisampulu yeplasma kuphela. Ayililo ixabiso lobungakanani okanye izinga lokunyuka kweDengue Uxinzelelo lwe-antibody lunokumiselwa ngolu vavanyo lomgangatho.
   2. 2. IDengue IgG/IgM Rapid Testiya kubonisa kuphela ubukho beDengue antibodies kumzekelo kwaye akufanelekanga ukuba isetyenziswe njengeyona ndlela yodwa yokuxilongwa kweDengue.
   3. 3. Ekuqaleni komkhuhlane, i-anti-Dengue IgM concentrations inokuba ngaphantsi kwamanqanaba abonakalayo. Kusulelo lokuqala, i-antibody ye-IgM-capture enzyme-edityaniswe ne-immunosorbent assay (MAC-ELISA) ibonise ukuba i-80% yezigulane zeDengue ezivavanyiweyo zibonise amanqanaba abonakalayo e-IgM antibody ngosuku lwesihlanu emva kosulelo, kwaye i-99% yezigulana zavavanywa ukuba zine-IgM. ngosuku lwe-10.⁵Kucetyiswa ukuba izigulane zivavanywe kweli xesha. 
   4. 4. Kusulelo lwesibini, iqhezu le-molar eliphantsi le-anti-Dengue IgM kunye neqhezu le-molar ephezulu ye-IgG esebenza ngokubanzi kwii-flaviviruses ebonakalisa izilwa-buhlungu.⁵ Isiginali ye-IgM inokuba buthathaka kwaye ukusabela komnqamlezo kwindawo yomgca we-IgG kunokuvela.
   5. 5. Umnqamlezo we-serological-reactivity kulo lonke iqela le-flavivirus (iDengue 1, 2, 3 & 4, St. Louis encephalitis, i-West Nile virus, i-encephalitis yaseJapan kunye neentsholongwane ze-yellow fever) zixhaphakile.^6,7,8Iziphumo ezilungileyo kufuneka ziqinisekiswe ngezinye iindlela.
   6. 6. Ubukho obuqhubekayo okanye ukungabikho kwama-antibodies akunakusetyenziselwa ukugqiba ukuphumelela okanye ukungaphumeleli kwonyango.
   7. 7. Iziphumo ezivela kwizigulane ezine-immunosuppressed kufuneka zitolikwe ngokuqaphela.
   8. 8. Njengazo zonke iimvavanyo zokuxilonga, zonke iziphumo kufuneka zitolikwe kunye nolunye ulwazi lwekliniki olukhoyo kugqirha.
   9. 9. Ukuba iziphumo zovavanyo zibi kwaye iimpawu zeklinikhi ziqhubeka, uvavanyo olongezelelweyo usebenzisa ezinye iindlela zeklinikhi ziyacetyiswa. Isiphumo esibi asikuthinteli nangaliphi na ixesha ukuba nokwenzeka kweDengue.
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