Ukukhawuleza kunye nokuthenjwa kokuzama ukuvavanywa kweMalariya P.F./pan antign

Intshayelelo

Imalariya ibangelwa yi-protozon eneeseli ezibomvu zabantu zegazi.1 Umbutho wezeMpilo weHlabathi loQinisekisa ukuba i-3.3 yezigidigidi yayisisifo seengcongconi ngo-2006, kwaye iphantse ibe sisigidi esinye ( Uninzi lwabantwana baseAfrika) babulawa kwesi sifo. Uhlalutyo olu-2 lwe microscopic ye-smears efanelekileyo kwaye i-smear yegazi elinqabileyo ibiyindlela esemgangathweni yokuchonga usulelo lweMalariya ngaphezulu kwenkulungwane. Ubuchule bunokwazi ukuxilongwa ngokuchanekileyo nangokuthembekileyo xa kusenziwa zii-microscopists ezinobuchule usebenzisa iiprotokholi ezichaziweyo. Isakhono semicroscopist kunye nokusetyenziswa kweenkqubo ezingqiniweyo nezichaziweyo, rhoqo zihambisa ezona mqobo zibalulekileyo ekufezekiseni ngokuchanekileyo ngokuchanekileyo kwe-microscopic. Nangona kukho umthwalo wokungena ngokudibeneyo nokwenza ixesha - Umsebenzi - Ubunzima - Izixhobo ezinokubangela ukusebenza kwe-microscopy ebonakalayo ekusebenziseni olu xinzelelo Itekhnoloji. Isifo seMalariya P.F / I-Pan Spere Isixhobo (igazi liphela) luvavanyo olukhawulezileyo ukuba lubone ubukho be-P.FEALCARUM - I-HRP ekhethekileyo - I-Alalcial Albolasum efumaneka kwi-P.FOLAMS (P.F), p I-.vivax (P.V), p.ovale (p.o) kunye p.ovale (P.O) kunye p Oluvavanyo lusebenzisa iGolloid iGolide yeColloid ukukhetha i-PF.F - I-PAL - I-Alal Adogens (P.F, P.O kunye ne-P.M) kwigazi liphela.

Izixhobo

Izixhobo ezibonelelweyo

Izinto ezifunekayo kodwa azibonelelwanga

Inkqubo

Vumela isixhobo sovavanyo, i-buffer, i-buffer, kunye / okanye ilawula ukulingana kubushushu begumbi (15 - 30 ° C) ngaphambi kovavanyo.

Susa isixhobo sovavanyo kwingxowa ye-foil kwaye uyisebenzise ngokukhawuleza. Iziphumo ezilungileyo ziya kufumaneka ukuba i-assay yenziwe ngaphakathi kwiyure enye.

Beka isixhobo sovavanyo kumphezulu ococekileyo kunye nenqanaba. Dlulisa inqaku lomgangatho okanye umbhobho:

Ukusebenzisa ipipette: Dlulisela i-10 mL ngegazi liphela, igazi elipheleleyo (Jonga umfanekiso ① ngezantsi). Khusela amaqamza omoya kwi-W1. Ekupheleni kwemizuzu emi-5, yongeza i-1 yehla epheleleyo ye-buffer kwi-W1 (Jonga umfanekiso ③ ngezantsi).

Ukusebenzisa i-pimette yokulahla inkunkuma: Bamba umbhobho ngokuthe nkqo; Zoba i-curimeny ukuya kumgca wokugcwalisa njengoko ubonisiwe kumzekeliso ① ngezantsi. Dlulisa inqaku le-S1 lesixhobo sovavanyo, emva koko wongeza amaconsi ama-3 apheleleyo e-buffer ukuya kwi-W2 kwaye uqalise ixesha. Khusela amaqamza omoya kwi-W1. Ekupheleni kwemizuzu emi-5, yongeza i-Buffer epheleleyo ye-buffer ukuya kwi-W1 (Jonga umfanekiso ③ ngezantsi).

3. Lindela umgca owombala ovele. Isiphumo kufuneka sifundwe kwimizuzu eli-15. Suku

Tolika isiphumo emva kwemizuzu engama-20.

Ukutolikwa kweziphumo

(Nceda ujonge kumzekeliso ongentla)

I-Hous: * Imigca emibini okanye emithathu eyahlukileyo.

1. I-Falciparum okanye usulelo oluxubileyo lwesilamu:Umgca omnye uyavela kwingingqi yolawulo, umgca omnye uvela kwingingqi yePante kunye nomgca omnye ubonakala kwingingqi ye-PF.F.
2. Usulelo lweFalciparum: Umgca omnye uvela kwingingqi yolawulo, kwaye umgca omnye ubonakala kwingingqi ye-PF.F.

Ayisi - I-Falciparum Plasmodium Bosuleleko:Umgca omnye uvela kwingingqi yolawulo kunye nomgca omnye ubonakala kwingingqi yePante.

* Qaphela: Ubunzulu bemibala ye-PF.F okanye imigca yovavanyo ye-PAN inokuhluka ngokuxhomekeka kuxinzelelo lwee-adoppens, i-viz., II okanye i-Alddolase ekhoyo kwinqaku.

I-negative: inye kuphela umgca onombala kuphela ovela kwingingqi yolawulo.

Ayisebenzi: Umgca wolawulo uyasilela ukuvela. Ivolumu engonelanga okanye iindlela zenkqubo ezingachanekanga zezona zizathu zibalulekileyo zokusilela komgca. Phonononga le nkqubo kwaye uphinda uvavanyo ngesixhobo esitsha sovavanyo. Ukuba ingxaki iyaqhubeka, ukuyeka ukusebenzisa ikhithi yovavanyo kwangoko kwaye uqhagamshelane nomsasazi wakho wengingqi.

Kwipani ye-PAN: 99.20% (I-95% CI: 95.16%~> 99.99%)  

Kwi-PF.F:> 99.99% (I-95% CI: 93.02%~I-100,00%)

Ukuchaneka kwe-98.94% (I-95% CI:I-96.79%~I-99.79%) 

Ukuchaneka: i-99.15% (I-95% CI:I-97.7%~I-99.75%)