Feline bin virus anti yovavanyo olukhawulezayo

• Ukusetyenziswa kwenjongo

I-feline corona intsholongwane i-antiction anti yovavanyo lwekhasethi yokuvavanya ukuba ibone ubukho be-feline corona intsholongwane, ukubonelela ngokuchonga usulelo lwe-feline.

Ixesha le-Assay: 5 - Imizuzu eli-10

I-Supemen: I-Srium, iplasma okanye igazi liphela

• Umgaqo

Uvavanyo lwe-FCOV lusekwe kwi-Sandwich I-Sunocramatographic Stureal Areay.

• Iindawo zokuhlala kunye nezixhobo
• I-foil pouches (nganye iqulethe i-carsetteand enye ye-Castecant)
• I-Assay buffer
• I-capillary droppers
• Incwadana yemigaqo
• UkugcinwaKunye nokuzinza

Ikhithi inokugcinwa kubushushu begumbi (4 - 30 ° C). Ikhithi yovavanyo izinzile ngomhla wokuphelelwa (iinyanga ezingama-24) iphawulwe kwileyibhile yephakheji. Musa ukukhulula. Musa ukugcina ikhithi yovavanyo ngokukhanya kwelanga ngqo.

• Ukulungiswa okukhulu kunye nokugcinwa

1. Kuya kufuneka ifumaneke kwaye iphathwe njengezantsi.

• I-Serumor Plasma: Qokelela lonke igazi kwikati yesigulana, i-Centrifuyigrafi ukuba ifumane i-serum, okanye ibeke yonke igazi kwityhubhu enee-anticoants ukufumana i-anticoants ukufumana i-anticoants ukufumana i-anticoants ukufumana i-anticoants ukufumana iPlasma.
• Igazi liphela: Qokelela igazi elitsha lokusetyenziswa ngokuthe ngqo okanye lenza igazi elichasene ne-2 - 8 ℃.

2. Yonke imiqathango kufuneka ivavanywe kwangoko. Ukuba akunjalo ngoku, mabagcinwe kwi-2 - 8 ℃.

• Inkqubo yovavanyo
• Vumela zonke izixhobo, kubandakanya nesixhobo esikhankanyiweyo kunye novavanyo, ukubuyela kwakhona kwi-15 - 25 ℃ Ngaphambi kokuba ubaleke i-assay.
• Thatha i-chest forfrom i-foil pauch kwaye ubeke ngokuthe tye.

Ukusebenzisa i-capillary ye-capillary ukubeka i-8μl yesampulu yesampulu "yesixhobo sovavanyo. Emva koko phosa amaconsi ama-3 (i-Appropt. 90μl) ye-asfy buffer kwimngxunya yesampulu kwangoko.

• Tolika isiphumo kwi-5 - isiphumo se-10 emva kokuba imizuzu eli-10 ithathwa njengengasebenziyo.
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• • Ukutolikwa kweziphumo
  • IQHELEKO (+): ubukho bobabini "C" i-Counend Comter Zone "I-T" umgca, akukho ncedo lwe-T licacile okanye lungaqhelekanga.
  • I-HAIF (-): Kuphela kuvela kuphela umgca. Akukho mgca we-t.
  • Ayisebenzi: Akukho mgca unombala uvela kumda we-C. Akunandaba nokuba i-T LI ibonakala.
  • UKULUMKELA
  • Zonke iiRegednts kufuneka kubushushu begumbi ngaphambi kokuba isebenze.
  • Sukukhupha i-carsette yovavanyo kwingxowa yayo kude kufike kwangoko ngaphambi kokusebenzisa.
  • Sukusebenzisa uvavanyo ngaphaya komhla wayo wokuphelelwa.
  • Amacandelo kule khithi abe lulawulo oluphambili kuvavanywa njengeyunithi esemgangathweni ye-batch. Sukudibanisa izinto ezivela kwiinombolo ezahlukeneyo ezininzi.
  • Zonke iimodeli zinokuba losulelo olunokubakho. Kufuneka iphathwe ngokungqongqo ngokwemigaqo nemigaqo ngamazwe asekuhlaleni.
  • Umda

  I-ferine corona intsholongwane i-antiction anti yovavanyo lwe-vitro ye-vitro ye-vitro venti kuphela isetyenziswa kuphela. Zonke iziphumo kufuneka ziqwalaselwe ngolunye ulwazi lweklinikhi olukhoyo nogqirha wezilwanyana. Kucetyiswa ukufaka isicelo sendlela eqinisekisayo ngakumbi efana neblow yeNtshona xa iziphumo ezilungileyo ziqwalaselwa.