I-Giadia Lamlia Antign yovavanyo olukhawulezayo

Ukusetyenziswa kwenjongo

Uvavanyo lwe-Giaria Antign Uvavanyo olukhawulezileyo lwe-chromasomatographic le-chromassic ye-chromassic yokufumanisa ukwenziwa komgangatho wama-Giaria Antigen Antign kwi-Storeign Storen. Iziphumo zovavanyo zenzelwe ukunceda ekufumanekeni kwesifo sosulelo lwe-Giadia kwaye sijonge ukusebenza ngonyango.

Izinto

Izixhobo ezibonelelweyo

Izixhobo zovavanyo zalo

 Faka iphakheji

Imibhobho yokulahla

 Iityhubhu zengqokelela

Ukukhupha i-buffer

Izinto ezifunekayo kodwa azibonelelwanga

Isikhongozeli sokuqokelela

 ixesha

Inkqubo yovavanyo

Yiza novavanyo, iisampuli, kunye / okanye ulawulo kubushushu begumbi (15 - 30 ° C)ngaphambi kokusetyenziswa.

1. 1. Susa uvavanyo kwingxoxo yayo etywiniweyo, kwaye ubeke kwindawo ecocekileyo nenomgangatho. Faka ilebhile ifowuni kunye nokuchongwa kwesigulana okanye ukulawula. Ngenxa yeziphumo ezilungileyo i-ASAST kufuneka yenziwe ngaphakathi kwiyure enye.

2. I-2. Ukulungiswa okutsha

Unscrew the sample bottle, use the attached applicator stick attached on the cap to transfer small piece of stool (4-6 mm in diameter; approximately 50 mg – 200 mg) into the sample bottle containing specimen preparation buffer. Ngolwelo okanye uSemi - Izitulo eziqinileyo, zongeza i-100 i-chinaroliter yesitulo kwi-vial ngombhobho ofanelekileyo. Faka intonga kwibhotile kwaye uqinise ngakumbi. Umxube isampulu ye-stool kunye ne-buffer ngokucacileyo ngokucheba ibhotile kwimizuzwana embalwa.

3. 3. Inkqubo ye-Assay

3.1 Bamba isampuli yebhotile ngokuthe tye ngenqaku lencam kwicala lokuya kwicala lokuvavanywa, phuma kwincam.

3.2. Bamba ibhotile kwisikhundla esithe nkqo ngaphezulu kwesampulu yekhadi lovavanyo, uhambise amaconsi ama-3 (120 - i-70 μl) yesampulu yesitulo esihlanjelweyo kwimodeli (s) kwaye uqalise ixesha. Kulumkele ukubopha iibhubhu ze-air kwi-specian kakuhle (s), kwaye akongezi nasiphi na isisombululo kwisiphumo. Njengoko uvavanyo luqala ukusebenza, umbala uza kuqhutywa kwindawo yonke kwiziphumo embindini wesixhobo.

3.3. Lindela iqela lemibala (s) lokuvela. Funda isiphumo phakathi kwe-5 - Imizuzu eli-10. Isampulu eyomeleleyo enamandla inokubonisa kwangaphambili.

Musa ukutolika iziphumo emva kwemizuzu eli-10.

Njengoko uvavanyo luqala ukusebenza, umbala uza kuqhutywa kwindawo yonke kwiziphumo embindini wesixhobo.

Ukutolikwa kweziphumo

I-Honess: Iibhanti ezimbini ezinemibala zivela kwi-membrane.Ibhendi enye ivela kwingingqi yolawulo (c) kwaye enye ibhendi ivela kummandla wovavanyo (T).

I-negating: Ibhendi enye kuphela ivela kwingingqi yolawulo (c).Akukho bandi zibonakalayo zibonakalayo kwingingqi yovavanyo (t).

Ayisebenzi: Ibhendi yolawulo iyasilela ukuvela.Iziphumo ezivela kulo naluphi na uvavanyo olungavelisi ibhendi yolawulo ngexesha lokufunda elichaziweyo kufuneka lilahlwe. Nceda ujonge inkqubo kwaye uphinde uphinde ngovavanyo olutsha. Ukuba ingxaki iyaqhubeka, ukuyeka ukusebenzisa i-kit kwangoko kwaye uqhagamshelane nomsasazi wakho wengingqi.

PHAWULA:

1. Ubunzulu bombala kuMmandla wovavanyo (T) bungahluka ngokuxhomekeka kuxinzelelo lwehlalutyishini ekhoyo kwinqaku. Ke ngoko, nawuphi na umthunzi wombala kummandla wovavanyo kufuneka uthathwe njengento entle. Qaphela ukuba olu luvavanyo olufanelekileyo kuphela, kwaye awukwazi ukufumanisa ukugxininiswa kohlalutyiweyo ku-Speciamen. Ivolumu engonelanga, inkqubo yokusebenza engachanekanga okanye iimvavanyo eziphelelwe lixesha zezona zizathu zibalulekileyo zokungaphumeleli kwebhendi.

   1. UKUHLAZIYWA KOKUVAVANYA

   2. 1. I-Giadiya i-Giaria Antign yovavanyo lwenzelwe ukusetyenziswa kwe-vitro yokusebenzisa, kwaye kufuneka isetyenziselwe ukubonwa komgangatho we-Giadia.
   3. 2. Iziphumo zovavanyo kufuneka zisetyenziswe kuphela ukuvavanya isigulana ngemiqondiso kunye neempawu zesifo. Isifo esiqinisekileyo seklinikhi kufuneka senziwe kuphela ngugqirha emva kokuba yonke iKlinikhi yeKlinikhi iphononongwe.
   4. I-3. Iisampulu ezivela kwizigulana ezifumene amalungiselelo ee-antibodies ze-monoclon Ezo zinto zinokubangela iziphumo ezingezizo ezingezizo okanye ezibubuxoki.
   5. 4. Njengoko zonke iimvavanyo zokuxilonga, isifo esiqinisekisiweyo kufuneka senziwe kuphela ngugqirha emva kokuba kufundwe zonke izinto zekliniki kunye nelebhu.
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