I-Campylobacter Antigen Ukuhlolwa Okusheshayo

Ukusetshenziswa okuhlosiwe

I-Campylobarbacter Antigen Rapid Test iyi-Immunoassay esheshayo ye-Chromatographic yokutholwa kwe-campylobarbacter spp. I-Antigen ku-Human Stool Specimen. Imiphumela yokuhlolwa ihloselwe ukusiza ekuqaleni - Ukuxilongwa kwesiteji kwe-Campylobacterf Ukutheleleka kweziguli ezinezimpawu ze-gastroenteritis ezibangelwa yi-castrobacter.

Into uqobo lwayoS

Izinto ezinikeziwe

Izinto ezidingekayo kodwa azinikeziwe

UkuzamaIndlela yokwenza izinto

Letha izivivinyo, ama-specimentens, kanye / noma izilawuli zokushisa kwegumbi (15 - 30 ° C) ngaphambi kokusebenzisa.

1. Susa isivivinyo esikhwameni sayo esivalekile, bese uyibeka endaweni ehlanzekile, yezinga. Faka ilebuli insiza ngesineke noma sokulawula ukukhonjwa. Ngemiphumela emihle kakhulu i-Assay kufanele yenziwe kungakapheli ihora elilodwa.
2. Ukulungiselela

Susa ibhodlela lesampula, sebenzisa induku ye-provil enamathiselwe enamathiselwe kwi-cap ukudlulisa ucezu oluncane lwesitulo (4 - 6 mm ubukhulu; Ngoketshezi noma i-semi - izitulo eziqinile, engeza ama-microliters ayi-100 we-stool uye kwi-vial ene-pipette efanele. Faka esikhundleni induku ku-tube bese uqinisa ngokuphepha. Hlanganisa isampula ye-Stool nge-buff kahle ngokuxhawula ibhodlela imizuzwana embalwa.

3. Inqubo yokufa

3.1 Bamba ishubhu le-assay buffer light light ngephuzu le-tip libheke eceleni komqhudelwano wokuhlola, unqamule ithiphu.

3.2. Bamba ibhodlela endaweni ebheke phezulu ngaphezulu kwesampula kahle yekhadi lokuhlola, ukuletha amaconsi ama-3 (120 - 150 μl) wesampula ye-Stool exutshwe emnandi (amasampula) bese uqala isibali sikhathi.

Gwema ukudonsa ama-bubble air e-specimen (futhi ungangezi noma yisiphi isisombululo endaweni yasemphumela.

Njengoba isivivinyo siqala ukusebenza, umbala uzokuhamba ngaphesheya komphumela enkabeni yedivayisi.

3.3. Linda ama-band (s) anemibala avele. Funda umphumela phakathi kwe-5 - Imizuzu eyi-10. Isampula eqinile enhle ingakhombisa umphumela ngaphambili.

Ungahumushi umphumela ngemuva kwemizuzu eyi-10.

Njengoba isivivinyo siqala ukusebenza, umbala uzokuhamba ngaphesheya komphumela enkabeni yedivayisi.

Ukuhunyushwa kwemiphumela

Okuhle: Okubili Amabhendi anemibala avela ku-membrane. Ibhendi eyodwa ivela esifundeni sokulawula (c) kanti elinye ibhendi livela esifundeni sokuhlola (T).

Okungalungile: Ibhendi eyodwa kuphela enemibala evele esifundeni sokulawula (c).Alikho ibhande elinombala elibonakalayo elivela esifundeni sokuhlola (T).

Okungavumelekile: I-Control band yehluleka ukuvela.Imiphumela kusuka kunoma yikuphi ukuvivinya okungakhiqizi ibhendi yokulawula ngesikhathi esichaziwe sokufundwa kumele kulahlwe. Sicela ubuyekeze inqubo bese uphinda ngovivinyo olusha. Uma inkinga iqhubeka, yeka ukusebenzisa ikhithi ngokushesha bese uthinta umsabalalisi wakho wangakini.

Izici Zokusebenza

Ithebula: I-Campylobacter antigen ngokushesha vs isiko lesiko

Ukuzwela okuhlobene: 98.51% (95% CI: 91.25% ~> 99.99%)

Ukucaciswa okuhlobene: 97.89% (95% CI: 95.62% ~ 99.06%)

Ukunemba: 97.99% (95% CI: 96.02% ~ 99.05%)