Ukusetshenziswa okuhlosiwe
I-Chlamydia Antigen Rapid Test iyi-ImmunoasAssay esheshayo ye-Chromatographic yokutholwa okufanelekile kwe-Chlamydia Trachomatis Antigen ku-Swab yesibeletho sabesifazane kanye nabesilisa u-Urethral Swab specimen. Imiphumela yokuhlolwa ihloselwe ukusiza ekutholakaleni kokutheleleka kwe-chlamydia kubantu.
Ukubeka kafushane
I-Chlamydia Trachomatis iyimbangela evame kakhulu yokutheleleka ngokocansi emhlabeni. Ihlanganiswe nemizimba yakudala (ifomu elithathelwanayo) kanye nezinhlaka zokuphindisela (ifomu lokuphindaphinda), i-chlamydia trachomatis inokubumbana okuphezulu kanye nezinkinga ezimbi kakhulu zabesifazane nakuma-neonates. Izinkinga zokutheleleka nge-chlamydia kwabesifazane zifaka i-cervicitis, i-urethritis, endometritis, i-PID kanye nezehlakalo ezandisiwe zokukhulelwa kwe-ectopic nokuzala inzalo. Ukudluliswa okuthe mpo kwalesi sifo ngesikhathi sokuhlukaniswa okuvela kumama kuya ku-neonate kungaholela ekufakweni kwe-conjunctivitis pneumonia. Kwabesilisa, izinkinga zokutheleleka nge-chlamydia zibandakanya i-urethritis ne-epidedlitis. Cishe ama-70% abesifazane abanokutheleleka kwe-endocavil kanye nama-50% wamadoda anezifo ze-urethrold yi-asymptomatic.
I-Chlamydia Antigen Rapid Test isivivinyo esisheshayo sokuthola kahle i-chlamydia antigen kusuka kuma-spab wesibeletho sowesifazane wesibeletho.
Izinto zokwakha
Izinto ezinikeziwe
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· Amadivayisi wokuhlola agcwele ngawodwa |
· Amashubhu we-Extraction |
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· I-Swabs elahlekayo yesampula (isibeletho sowesifazane) |
· Amathiphu we-Dropper |
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· I-Extraction reagent 1 (0.2m Naoh) |
· I-WorkStation |
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· I-Extraction Reagent 2 (0.2 M HCI) |
· Faka iphakheji |
Izinto ezidingekayo kodwa azinikeziwe
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· Ama-Urethile Male Urethral Swabs |
· Umninisikhathi |
Inqubo yokuhlola
Vumela isivivinyo, ama-reagents, i-swab specimen, kanye / noma izilawuli ukufinyelela amazinga okushisa egumbi (15 - 30 ° C) ngaphambi kokuhlolwa.
- 1. Susa ikhasethi lokuhlola kusuka esikhwameni se-foil bese usisebenzisa kungakapheli ihora elilodwa. Imiphumela emihle izotholakala uma ukuhlolwa kwenziwa ngokushesha ngemuva kokuvula i-foil pauch.
- 2. Khipha i-chlamydia antigen ngohlobo lwe-specimen.
Ngowesifazane wesibeletho noma wesilisa u-Urethral Swab specimen:
- Bamba ibhodlela le-reagent 1 libheke phezulu bese wengeza u-5Amaconsi we-reagent 1(approx. 300μl) kwishubhu lokukhishwa. I-Reagent 1 ayinamibala. Ngokushesha faka i-swab, cindezela ezansi kweshubhu futhi ujikeleze i-swab izikhathi eziyi-15. Ake umeImizuzu emi-2.
- Bamba ibhodlela le-reagent 2 mpo engezaAmaconsi ayi-6 we-reagent 2(approx. 250μl) kwishubhu lokukhishwa. Isixazululo sizojika ngesikebhe. Cindezela ibhodlela le-tube futhi ujikeleze ama-swab amahlandla ayi-15 kuze kube yilapho isixazululo siguqule sicacile nge-tint encane eluhlaza noma eluhlaza okwesibhakabhaka. Uma i-swab inegazi, umbala uzophenduka ophuzi noma onsundu. Ake ume imizuzu eyi-1.
- Cindezela i-swab ngokumelene nohlangothi lweshubhu bese uhoxisa i-swab ngenkathi ucindezela ishubhu. Gcina uketshezi oluningi ku-tube ngangokunokwenzeka. Finyelela ithiphu le-dropper ngaphezulu kweshubhu lokukhishwa.
- 3. Beka ikhasethi lokuhlola endaweni ehlanzekile neleveli. Faka amaconsi amathathu aphelele wesisombululo esikhishwe (cishe. 100μl) Emithonjeni ngayinye ye-cassen ye-cassette yokuhlola, bese uqala isibali sikhathi. Gwema ukudonswa kwama-bubble air e-specimen kahle.
- 4. Lindela umugqa onemibala ukuze uvele.Funda umphumela ku-10imizuzu;Ungahumushi umphumela ngemuva kwemizuzu engama-20.
Qaphela:Kunconywa ukusebenzisa i-buffer kungakapheli izinyanga eziyi-6 ngemuva kokuvula i-vial.
Ukuhunyushwa kwemiphumela
Okuhle: Amabhendi amabili anemibala avela ku-membrane. Ibhendi eyodwa ivela esifundeni sokulawula (c) kanti elinye ibhendi livela esifundeni sokuhlola (T).
Okungalungile: Ibhendi eyodwa kuphela enemibala evele esifundeni sokulawula (c).Alikho ibhande elinombala elibonakalayo elivela esifundeni sokuhlola (T).
Okungavumelekile: I-Control band yehluleka ukuvela.Imiphumela kusuka kunoma yikuphi ukuvivinya okungakhiqizi ibhendi yokulawula ngesikhathi esichaziwe sokufundwa kumele kulahlwe.
Sicela ubuyekeze inqubo bese uphinda ngovivinyo olusha. Uma inkinga iqhubeka, yeka ukusebenzisa ikhithi ngokushesha bese uthinta umsabalalisi wakho wangakini.
Qaphela:
- 1. Ukuqina kombala esifundeni sokuhlola (t) kungahluka ngokuya ngokuhlaziywa kokuhlaziywa okukhona ku-specimen. Ngakho-ke, noma yimuphi umthunzi wombala esifundeni sokuhlola kufanele uthathwe njengento efanele. Qaphela ukuthi lokhu kungukuhlola okufanelekile kuphela, futhi akunakunquma ukugcwala kokuhlaziywa kwezinhlamvu.
- 2. IVolumu Enganele Enganele, inqubo engalungile yokusebenza noma izivivinyo eziphelelwe yisikhathi yizizathu zokulawula ukwehluleka kwebhendi.
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Ukulinganiselwa kokuhlolwa
- 1. I-ChlamyDiaantigen Ukuhlolwa Okusheshayo kochwepheshe eVitro Ukusetshenziswa kokuxilonga, futhi kufanele kusetshenziselwe kuphela ukutholwa kokufaneleka kokutheleleka kwe-chlamydia yabantu.
- 2. Umphumela wokuhlola kufanele usetshenziselwe kuphela ukuhlola isiguli esinezimpawu nezimpawu zesifo. Ukuxilongwa kwemitholampilo okuchazayo kufanele kwenziwe kuphela ngudokotela ngemuva kokuhlolwa konke kwezokwelapha kanye nokutholwa kwelebhu.
- 3. Njenganoma iyiphi i-antibodies yegundane esebenzisa igundane, kungenzeka ukuthi kuphazanyiswe yi-anti yabantu - ama-antibodies egundane (Hama) ezinsweni. Izinhlobo ezivela ezigulini ezithole amalungiselelo ama-antibodies e-monoclonal yokuxilongwa noma ukwelashwa kungaqukatha uHama. Izinhlobo ezinjalo zingadala imiphumela emibi noma engamanga.
4. Njengoba konke ukuhlolwa kokuxilonga, ukuxilongwa okuqinisekisiwe kufanele kwenziwe kuphela udokotela ngemuva kokuthola ukuhlolwa kokutholakele kwemitholampilo.
