I-Closerpidium Efficile Antigen Rapid Test

Ukusetshenziswa okuhlosiwe

I-Closerpidium Effile(GDH) I-Antigen Rapid Test iyi-Immunoassay esheshayo ye-Chromatographic yokutholwa kwe-glutamate dehydrogenase ku-Human Stool specimen. Imiphumela yokuhlolwa yenzelwe ukusiza ukuthola i-antibiotic - I-Colitis ehlobene ne-Colitis ne-Pseudomembranous Colitis ebangelwa ukutheleleka ngeI-Closerpidium Effile.

Isimiso

Usebenzisa umgomo weColloidal Gold Immunochrofophrography, imbuzi anti - I-Mouse Polyclonal Antibody (Series C) negundane anti -I-Closerpidium EffileI-Monoclonal antibody yayihlanganiswe kwi-Nitrate Cellulose Film. Ama-antibodies e-mouse monoclonal ukuzeC. FIFICILEzalungiswa ngamapuleti egolide ngamalebula wegolide le-colloidal. Lapho kuhlolwa isampula elifanele,I-Closerpidium EffileI-Antigen kwisigaxa esibopha igundaneI-Closerpidium EffileI-Monoclonal antibody ku-pad yegolide, yakha inkemba ehamba nolwelwesi nge-chromatography. Ngemuva komugqa wokutholwa, kwakha isandwich eyinkimbinkimbi nge-pre - i-antibod ye-pre - ehlanganiswe nembuzi i-antibody yombala, i-mouse igg polyclonal antilody yomugqa wokulawulwa kombala, amasampula angemuhle umbala wokubonisa kuphela kulayini wokulawulwa kwekhwalithi.

Inqubo yokuhlola

Letha izivivinyo, ama-specimentens, kanye / noma izilawuli zokushisa kwegumbi (15 - 30 ° C)ngaphambi kokusebenzisa.

1. Susa isivivinyo esikhwameni sayo esivalekile, bese uyibeka endaweni ehlanzekile, yezinga. Faka ilebuli insiza ngesineke noma sokulawula ukukhonjwa. Ngemiphumela emihle kakhulu i-Assay kufanele yenziwe kungakapheli ihora elilodwa.

2. Ukulungiselela okucacisiwe:

QAPHELA I-CAP ye-Specimen Collection Tube, bese ugwaza ngokungahleliwe induku yesampula ku-smool specimen okungenani amasayithi amathathu ahlukene ukuqoqa cishe ama-stools (okulingana no-1/4 wethanga). Bamba amashubhu wokuqoqa ama-specimen nge-buffer ekhishwe ngokuthe thini, faka induku yesampula, cwilisa phansi kweshubhu. Hlanganisa isampuli ye-stool nge-buff kahle ngokuxhawula ibhodlela ngemizuzwana embalwa.

3. Inqubo yokufa:

 Qinisekisa i-cap ku-tube ye-specimen collection, bese unikina i-specimen Collection tube ngamandla ukuze uxuse okuhlotshisiwe kanye nesiphazamiso sokukhishwa. Shiya ishubhu lodwa imizuzu emi-2.

 Susa isembozo esincane phezulu.

 Bamba ibhodlela endaweni ebheke phezulu ngaphezulu kwesampula kahle yedivayisi yokuhlola, ukuletha amaconsi ama-3 (cishe ama-90μL) wesampula ye-stool exutshwe kahle (amasampula) wesampula futhi aqale isibali sikhathi.

Qaphela:Gwema ukudonsa ama-bubble air e-specimen (futhi ungangezi noma yisiphi isisombululo esifundeni umphumela.

Njengoba isivivinyo siqala ukusebenza, umbala uzokuhamba uzuze khona isifunda somphumela enkabeni yedivayisi.

 Lindela amabhendi anemibala ukuze avele. Funda umphumela phakathi kwemizuzu engu-5 - 10. Isampula eqinile enhle ingakhombisa umphumela ngaphambili. Ungahumushi umphumela ngemuva kwemizuzu engu-15. Njengoba ukuhlolwa kuqala ukusebenza, umbala uzokwenza

suka kwisifunda somphumela enkabeni yedivayisi.

Ukuhunyushwa kwemiphumela

Okuhle (+):Kuvela amabhendi amabili abomvu onsomi. Enye itholakala esifundeni sokutholwa (t), enye itholakala esifundeni sokulawula ikhwalithi (C).

Qaphela:I-band ebomvu ebubende esifundeni sokutholwa (T) ingakhombisa into yombala omnyama nokhanyayo. Kodwa-ke, ngesikhathi sokubukwa okucacisiwe isikhathi, kungakhathalekile umbala webhendi, iqembu elibuthakathaka kakhulu

kufanele ihunyushwe njengomphumela omuhle.

Okubi (-):Kuvela i-Purple Red Band ku-Izinga Lokulawula Ikhwalithi (C). Awekho amabhendi abomvu ansomi atholakala esifundeni sokutholwa (T). Umphumela ongemuhle uyakhombisa chaI-Closerpidium EffileUkutheleleka.

Okungavumelekile:Alikho i-band ebomvu ebubende kwisifunda sokulawulwa kwekhwalithi (C). Kukhombisa ukusebenza okungalungile noma ukuwohloka kwesivivinyo. Kulokhu, funda imiyalo ngokucophelela futhi futhi uphinde uphinde uhlole ngovivinyo olusha. Uma inkinga

uphikelela, kufanele uyeke ngokushesha ukusebenzisa inombolo ye-batch bese uthinta umphakeli wakho wendawo

Ukukhawulelwa

1. 1. TheI-Closerpidium Effile(GDH) Ukuhlolwa okusheshayo kwe-Antigen kungochwepheshein iqhintaUkuthola Ukuxilonga, Futhi Kufanele Kusetshenziselwe Ukutholwa Kwomuntu KuwoI-Closerpidium Effile.

2. 2. Umphumela wokuhlola kufanele usetshenziselwe kuphela ukuhlola isiguli esinezimpawu nezimpawu zesifo. Ukuxilongwa kwemitholampilo okuchazayo kufanele kwenziwe kuphela ngudokotela ngemuva kokuhlolwa konke kwezokwelapha kanye nokutholwa kwelebhu.

3. 3. Njenganoma iyiphi i-antibodies yegundane esebenzisa igundane, kungenzeka ukuthi kuphazanyiswe yi-anti yabantu - ama-antibodies egundane (Hama) ezinsweni.

4. Izinhlobo zezempi ezivela ezigulini ezithole amalungiselelo ama-antibodies e-monoclonal yokuxilongwa noma ukwelashwa kungaqukatha uHama. Izinhlobo ezinjalo zingadala imiphumela emibi noma engamanga.

4. I-5. Njengoba konke ukuhlolwa kokuxilonga, ukuxilongwa okuqinisekisiwe kufanele kwenziwe kuphela udokotela ngemuva kokutholwa konke okutholakele emtholampilo nakwelabhoratri.
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