Ukuhlolwa kwe-Lyme antibody okusheshayo

Ukusetshenziswa okuhlosiwe

I-Lyme Borrelia IGG / IGM Test Test ye-Chromatographic i-Immunoassay esheshayo yokutholwa kwe-antibodies ye-IGG ne-IGM ku-Borrelia SPP. egazini lonke yabantu, i-serum noma i-plasma specimen.

Ukuqalisa

Isifo seLyme, esaziwa nangokuthi uLyme Borreliosis, yisifo esithathelwanayo esidalwa ngamagciwane eBorrelia SPP. okusatshalaliswa yimikhaza. Isibonakaliso esivame kakhulu sokutheleleka yindawo ekhulayo yokubomvu esikhunjeni, esaziwa ngokuthi ama-erythema migran, oqala endaweni yokuluma umkhaza cishe ngesonto ngemuva kokuba sekwenzekile.1 Cishe 25 - I-50% yabantu abathelelekile ayikhuli ukuqubuka. Ezinye izimpawu zakuqala zingafaka umkhuhlane, ikhanda futhi uzizwe ukhathele. Uma kungalashwa, izimpawu zingafaka ukulahleka kwekhono lokuhambisa izinhlangothi zombili zobuso, izinhlungu ezihlangene, ubuhlungu bekhanda obukhulu ngokuqina kwentamo, noma ukuqina kwenhliziyo, phakathi kwabanye. Izinyanga eminyakeni eyalandela, iziqephu eziphindaphindwayo zobuhlungu obuhlangene nokuvuvukala kungenzeka. Ngezikhathi ezithile, abantu bathuthukisa izinhlungu zokudubula noma ukuqubuka ezihlahleni nasemilenzeni. Naphezu kokwelashwa okufanele, cishe ama-10 kuye kwangu-20% abantu athuthukisa izinhlungu ezihlanganisiwe, izinkinga zememori, futhi uzizwe ukhathele okungenani izinyanga eziyisithupha.

Isifo seLyme sidluliselwa kubantu ngokuluma kwemikhaza enegciwane lefode. Imvamisa, umkhaza kumele unamathiselwe amahora angama-36 kuye kwangama-48 ngaphambi kokuba amagciwane asakazeke. ENyakatho Melika, iBorrelia Burgdorferi naseBorrelia Mayonii yizimbangela. EYurophu nase-Asia, iBacteria Borrelia Afzelii neBolllelia Garinii nazo zibangela lesi sifo. Lesi sifo asibonakali sitholwa phakathi kwabantu, ezinye izilwane, noma ngokudla. Ukuxilongwa kususelwa ekuhlanganiseni kwezimpawu, umlando wokuvezwa kokhayakha, futhi mhlawumbe uhlole amasosha omzimba athile egazini. Ukuhlolwa kwegazi kuvame ukungahambi kahle ezigabeni zokuqala zalesi sifo. Ukuhlolwa kwemikhaza ngakunye akukusebenzi ngokujwayelekile.I-Lyme Borrelia IGG / IGM Ukuhlolwa okusheshayo kuwukuhlola okusheshayo okusebenzisa inhlanganisela yeBorrelia antigen enemibala enemibala yokutholwa kwe-IGG ne-IGM eBorrelia SPP. ama-antibodies egazini lonke, i-serum, noma i-plasma.

Indlela yokwenza izinto

Vumela idivaysi yokuhlola, i-specimen, buffer, kanye / noma izilawuli ukufinyelela amazinga okushisa egumbi (15 30 ° C) ngaphambi kokuhlolwa.

1. Letha isikhwama sokushisa segumbi ngaphambi kokuvula. Susa idivaysi yokuhlola kusuka esikhwameni esivaliwe bese uyisebenzisa ngokushesha okukhulu.
2. Beka idivaysi yokuhlola endaweni ehlanzekile neleveli.

IngomaneAma-serum noma ama-plasma ama-plasma: 

Bamba i-dropper mpo, udwebe ama-specimenkuze kubeGcwalisa umugqa (Cishe u-10 ul), bese udlulisela ama-specimen ku-specimen kahle (ama) wedivayisi yokuhlola, bese wengeza amaconsi ama-2 we-buffer (cishe ama-80 ml) bese uqala isibali sikhathi. Bheka umfanekiso ngezansi. Gwema ukudonswa kwama-bubble air e-specimen.

IngomaneIgazi eliphelele (i-thonipuncture / Fingerstick):

Ukuze usebenzise i-dropper: bamba i-dropper mpo, udwebe ama-specimen0.5 - 1 cm ngaphezulu komugqa wokugcwalisa, futhi udlulise amaconsi ama-2 egazi lonke (cishe ama-20 μl) ku-specimen kahle (ama-specimen kahle wedivayisi yokuhlola, bese engeza amaconsi ama-2 we-buffer (cishe ama-80 ul) bese uqala isibali sikhathi. Bheka umfanekiso ngezansi.

Ukuze usebenzise i-micropipette: i-pipette kanye nokukhipha ama-20

3. Linda umugqa onemibala ukuze uvele. Funda imiphumela ngamaminithi ayi-10.Ungachazela umphumela ngemuva kwemizuzu engama-20.

Ukuhunyushwa kwemiphumela