I-Cryptospidium antigen ngokushesha okusheshayo

Ukusetshenziswa okuhlosiwe

I-CryptoSkoridium Parvum Antigen Rapid Test iyi-Immunoassay esheshayo ye-Chromatographic yokutholwa kwe-cryplospidium parvum antigen ku-Human Stool Specimen. Imiphumela yokuhlolwa ihloselwe ukusiza ekutholakaleni kokutheleleka kwe-cryplospidium parvum kanye nokubheka ukusebenza kahle kokwelashwa kwezokwelapha.

Ukuqalisa

I-Crypposporididiosis yisifo esithathelwanayo esidalwa yi-cryplospidium parvum, esingenzeka ngezikhathi ezithile esibangelwa ezinye izinhlobo ze-cryplospidium. Izimpawu zivame ukuvela cishe ezinsukwini eziyi-7 ngemuva kokutheleleka, kufaka phakathi ubuhlungu besisu, uhudo lwamanzi, ukugabha nomkhuhlane. Izimpawu zeziguli eziningi zihlala izinsuku ezi-6 kuye kwezingu-10, kepha zingahlala amasonto ambalwa. Iziguli ezinezinkinga zamasosha omzimba, njengokutheleleka, kungaba kubi kakhulu futhi ngisho nokuphila - ukusongela. Kusukela ngombiko osemthethweni ngo-1976, kutholakele ukuthi lesi sifo sisabalele, futhi kungukugcwala okuvamile kwesifo sohudo sabavakashi. Kuneziguli eziningi ezine-AIDS eziyinkimbinkimbi nalesi sifo.

Inqubo yokuhlola

Letha izivivinyo, ama-specimentens, kanye / noma izilawuli zokushisa kwegumbi (15 - 30 ° C) ngaphambi kokusebenzisa.

1. Susa isivivinyo esikhwameni sayo esivalekile, bese uyibeka endaweni ehlanzekile, yezinga. Faka ilebuli insiza ngesineke noma sokulawula ukukhonjwa. Ngemiphumela emihle kakhulu i-Assay kufanele yenziwe kungakapheli ihora elilodwa.
2. Ukulungiselela

Susa ibhodlela lesampula, sebenzisa induku yesicelo enamathiselwe enamathiselwe kwi-cap ukudlulisa ucezu oluncane lwesitulo (4 - 6 mm ubukhulu; Ngoketshezi noma i-semi - izitulo eziqinile, engeza ama-microliters ayi-100 we-stool uye kwi-vial ene-pipette efanele. Faka esikhundleni induku ebhodleleni bese uqinisa ngokuphepha. Hlanganisa isampula ye-Stool nge-buff kahle ngokuxhawula ibhodlela imizuzwana embalwa.

3. Inqubo yokufa

3.1 Bamba i-BOTTLE BOTTLE eqondile ngephuzu le-TIP libheke eceleni kude nomqhubi wokuhlola, unqamule ithiphu.

3.2. Bamba ibhodlela endaweni ebheke phezulu ngaphezulu kwesampula kahle yekhadi lokuhlola, ukuletha amaconsi ama-3 (120 - 150 μl) wesampula ye-Stool exutshwe emnandi (amasampula) bese uqala isibali sikhathi.

Gwema ukudonsa ama-bubble air e-specimen (futhi ungangezi noma yisiphi isisombululo endaweni yasemphumela.

Njengoba isivivinyo siqala ukusebenza, umbala uzokuhamba ngaphesheya komphumela enkabeni yedivayisi.

3.3. Linda ama-band (s) anemibala avele. Funda umphumela phakathi kwe-5 - Imizuzu eyi-10. Isampula eqinile enhle ingakhombisa umphumela ngaphambili.

Ungahumushi umphumela ngemuva kwemizuzu eyi-10.

Njengoba isivivinyo siqala ukusebenza, umbala uzokuhamba ngaphesheya komphumela enkabeni yedivayisi.

Ukuhunyushwa kwemiphumela

Okuhle: Okubili Amabhendi anemibala avela ku-membrane. Ibhendi eyodwa ivela esifundeni sokulawula (c) kanti elinye ibhendi livela esifundeni sokuhlola (T).

Okungalungile: Ibhendi eyodwa kuphela enemibala evele esifundeni sokulawula (c).Alikho ibhande elinombala elibonakalayo elivela esifundeni sokuhlola (T).

Okungavumelekile: I-Control band yehluleka ukuvela.Imiphumela kusuka kunoma yikuphi ukuvivinya okungakhiqizi ibhendi yokulawula ngesikhathi esichaziwe sokufundwa kumele kulahlwe. Sicela ubuyekeze inqubo bese uphinda ngovivinyo olusha. Uma inkinga iqhubeka, yeka ukusebenzisa ikhithi ngokushesha bese uthinta umsabalalisi wakho wangakini.

1. IKHWALITHI YOKULAWULA

   • Izilawuli zenqubo zangaphakathi zifakiwe esivivinyweni. Ibhendi enemibala ebonakala esifundeni sokulawula (C) ibhekwa njengethuluzi elifanelekile lezinqubo, eliqinisekisa ivolumu eyanele lenqubomgomo kanye necebo elifanele lezinqubo.
   • Izilawuli zangaphandle azihlinzekwe ngaleli khithi. Kunconywe ukuthi izilawuli ezinhle nezingezinhle zihlolwe njengomkhuba omuhle welabhoratri ukuze uqinisekise inqubo yokuhlola futhi uqinisekise ukusebenza okufanele kokuhlola.

Ukulinganiselwa kokuhlolwa

1. I-TheCryprySporpidium Parvum Antigen Rapige Test yenzelwe ukusetshenziswa kwe-vitro Diagnostic Ukusetshenziswa, futhi kufanele isetshenziselwe kuphela ukutholwa kwe-Cryplospidium ye-Human ParVum.
2. Umphumela wokuhlola kufanele usetshenziswe kuphela ukuhlola isiguli esinezimpawu nezimpawu zesifo. Ukuxilongwa kwemitholampilo okuchazayo kufanele kwenziwe kuphela ngudokotela ngemuva kokuhlolwa konke kwezokwelapha kanye nokutholwa kwelebhu.
3. Njenganoma iyiphi i-antibodies esebenzisa igundane esebenzisa igundane, kungenzeka ukuthi kuphazanyiswe yi-anti yabantu - ama-antibodies asegundane (Hama) ezinsweni. Izinhlobo ezivela ezigulini ezithole amalungiselelo ama-antibodies e-monoclonal yokuxilongwa noma ukwelashwa kungaqukatha uHama. Izinhlobo ezinjalo zingadala imiphumela emibi noma engamanga.

• Njengoba konke ukuhlolwa kokuxilonga, ukuxilongwa okuqinisekisiwe kufanele kwenziwe kuphela udokotela ngemuva kokutholaziwe konke okutholakele komtholampilo nakwelabhoratri.