I-Zika LGG / IGM ne-NS1 Combo Test
Ukusetshenziswa okuhlosiwe
I-Zika Virus IGG / IGM ne-NS1 Combo Test iyi-Immunoassay esheshayo ye-Chromatographic yokutholwa okufanelekile kwe-Zika Virus NS1 Antigen kanye ne-IGG / IGM Antibodies kuZika Virus egazini lonke yomuntu, i-serum noma i-plasma specimen.
Izinto zokwakha
Izinto ezinikeziwe
- Amadivayisi wokuhlola
- I-Dropper ebonakalayo elahlekile
- Faka iphakheji
Izinto ezidingekayo kodwa azinikeziwe
- Isitha sokuqoqwa kweqoqo
- Ilanseet
- I-centrifuge (ye-plasma kuphela)
- Umninisikhathi
Inqubo yokuhlola
Vumela idivaysi yokuhlola, i-specimen, buffer, kanye / noma izilawuli ukulingana nokushisa kwegumbi (15 - 30 ° C) ngaphambi kokuhlolwa.
- Letha isikhwama sokushisa segumbi ngaphambi kokuvula. Susa idivaysi yokuhlola kusuka esikhwameni esivaliwe bese uyisebenzisa ngokushesha okukhulu.
- Beka idivaysi yokuhlola endaweni ehlanzekile neleveli.
Igciwane le-zika IGG / IGM Ingxenye
Okwe-serum noma ama-plasma acembu:
Bamba i-dropper mpo, udwebe ama-specimen ufike kulayini wokugcwalisa (cishe ama-5 μL), bese udlulisela ama-specimen kwi-specimen kahle (bese ufaka amaconsi ama-3 we-buffer (cishe ama-90 ml) bese uqala isibali sikhathi . Bheka umfanekiso ngezansi. Gwema ukudonswa kwama-bubble air e-specimen.
Igazi eliphelele (i-venipuncture / Fingerchick) ama-specimentens:
Ukuze usebenzise i-dropper we-buffer (cishe 90 ul) bese uqala isibali sikhathi. Bheka umfanekiso ngezansi.
Ukuze usebenzise iMicropipette: I-PIPET NOKUFINYELELA I-10 μL yegazi lonke kuya ku-specimen kahle (s) kahle (bese wengeza amaconsi ama-3 we-buffer (cishe ama-90 μL) bese uqala isibali sikhathi. Bheka umfanekiso ngezansi.
Igciwane le-zika Ingxenye ye-NS1
Ukuze usebenzise i-dropper: gcwalisa iplastiki dropper nge-specimen. Ukubamba i-dropper mpo, ukhiphe amaconsi ama-2 (cishe ama-60μL) we-plasma / serum specimen noma amaconsi ama-3 (cishe ama-90μL) wesampula aqinisekise ukuthi akukho bub Bub Bubble.
QAPHELA: Faka i-1 yehla lwe-saline noma i-phosphate - I-saline buffer (ama-buffers ajwayelekile asetshenziswa emtholampilo anikezwe ku-kit) ku-flow mess uma udlula imizuzwana eyi-30. .
- Linda umugqa onemibala ukuze uvele. Funda imiphumela ngemizuzu eyi-10. Ungahumushi umphumela ngemuva kwemizuzu engu-15.
Ukuhunyushwa kwemiphumela
Igciwane le-zika IGG / IGM Ingxenye
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IgG okuhle:* Umugqa onemibala esifundeni sokulawula (C) uvela, futhi umugqa onemibala uvela esifundeni somugqa we-G. Umphumela ulungele ukufunwa kwegciwane le-zika - |
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IgM:* Umugqa onemibala esifundeni sokulawula (C) uvela, futhi umugqa onemibala uvela esifundeni se-test line M. Umphumela ubambile nge-Zika Virus Virus Virus Eqondile - |
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IgG namigM:* Umugqa onemibala esifundeni sokulawula (C) uvela, futhi imigqa emibili enemibala kufanele ivele ku-Test Line Region G noM. Ukuqina kombala kwemigqa akudingeki ukuthi kufane. Umphumela ulungele ama-antibodies we-IGG ne-IGM futhi ukhombisa ukutheleleka ngegciwane lesibili le-zika. |
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-Phikisayo: Umugqa onemibala esifundeni somugqa wokulawula (c)aI-P Ppears. Ayikho umugqa ovela ezifundeni zomugqa wokuhlola 1 noma 2 (T1 noma T2). |
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Okungavumelekile: Umugqa wokulawula (c) ehlulekaaijunda. IVUFL ye-Buffer Volumery enganele noma amasu angalungile enqubo yizo izizathu ezingenzeka zokwehluleka komugqa wokulawula. Buyekeza inqubo bese uphinda inqubo ngedivayisi entsha yokuhlola. Uma inkinga iqhubeka, yeka ukusebenzisa ikhithi yokuhlola ngokushesha bese uthinta umsabalalisi wakho wangakini. |
Igciwane le-zika Ingxenye ye-NS1
Okuhle:Zombili amabhendi we-C no-T avela esifundeni nasekulingweni.
Okungalungile:Umugqa owodwa kuphela onemibala ovela esifundeni sokulawula.
Okungavumelekile:Umugqa wokulawula wehluleka ukuvela. IVolumu enganele noma amasu angalungile emisebenzini ngezizathu ezinokwenzeka zokulawulwa komugqa wokulawula. Buyekeza inqubo bese uphinda isivivinyo ngedivayisi entsha yokuhlola. Uma inkinga iqhubeka, yeka ukusebenzisa ikhithi yokuhlola ngokushesha bese uthinta umsabalalisi wakho wangakini.
